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Bovine Osteopontin (OPN) Protein

Bio-Techne includes R&D Systems | Catalog # 109-OP

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Carrier Free
109-OP-050/CF

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With Carrier
109-OP-050

Key Product Details

Source

Bovine Milk

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Bovine milk-derived Osteopontin/OPN protein

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

SDS-PAGE

60 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Hu, D.D. et al. (1995) J. Biol. Chem. 270:26232.
When 1 x 105 cells/well are added to a Bovine Osteopontin/OPN coated plate, cell adhesion is enhanced in a dose-dependent manner after 1 hour incubation at 37 °C.  The ED50  for this effect is 0.06-0.36 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 109-OP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 109-OP/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Osteopontin/OPN

Osteopontin (OPN, also known as early T lymphocyte activation protein 1, bone sialoprotein 1, and secreted phosphoprotein 1), is a secreted, highly acidic,
calcium-binding, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels, and various tumor tissues. Many cell types can express OPN in response to activation by cytokines, growth factors, or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, tumor metastasis, and cerebral ischemia (1, 2). Bovine OPN cDNA encodes a 278 amino acid (aa) residue precursor with a 16 aa signal peptide that is cleaved to yield a 262 aa mature protein with an integrin binding sequence (RGD), a thrombin cleavage site and N- and O-glycosylation sites. Human, mouse, rat, porcine, and bovine OPN share approximately 40%-80% aa sequence identity. OPN has been shown to bind to different cell types through RGD-mediated interaction with Integrins alphav beta1, alphav beta3, alphav beta5, and non-RGD-mediated interaction with CD44 and Integrins alpha8 beta1 or alpha9 beta1. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells, and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3, and MMP-7. The functions of OPN in a variety of cell types are modified as a result of proteolytic cleavage (3, 4).

References

  1. Sodek, J. et al. (2006) J. Dent. Res. 85:404.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
  4. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Long Name

Secreted Phosphoprotein 1 [BNSP]

Alternate Names

Eta-1, OPN, Spp1

Entrez Gene IDs

6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine)

Gene Symbol

SPP1

Additional Osteopontin/OPN Products

Product Documents for Bovine Osteopontin (OPN) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Bovine Osteopontin (OPN) Protein

For research use only

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