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Bacterial Thermolysin Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 3097-ZN

R&D Systems, part of Bio-Techne
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3097-ZN-020

Key Product Details

Source

G. stearothermophilus

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Geobacillus stearothermophilus-derived Thermolysin protein

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.

Reviewed Applications

Read 4 reviews rated 4.8 using 3097-ZN in the following applications:

Formulation, Preparation and Storage

3097-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Thermolysin

Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.

Protease Catalog # [Thermolysin] Inhibitor
rhADAM8 1031-AD 0.75 μg/mL Phosphoramidon
rhEnterokinase 1585-SE 1.58 μg/mL 1, 10-Phenanthroline
rhFactor VII 2338-SE 6.25 μg/mL 1, 10-Phenanthroline
rhFactor XI 2460-SE 10 μg/mL EDTA
rhHGF Activator 1514-SE 4.3 μg/mL 1, 10-Phenanthroline
rhKallikrein 1 2337-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 3/PSA 1344-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 4 1719-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 7 2624-SE 10 μg/mL EDTA
rhKallikrein 11 1595-SE 10 μg/mL EDTA
rhKallikrein 14 2626-SE 10 μg/mL EDTA
rmMast Cell Protease-11 2857-SE 0.2 μg/mL 1, 10-Phenanthroline
rhPlasma Kallikrein 2497-SE 10 μg/mL EDTA
rmPlasma Kallikrein 2498-SE 10 μg/mL EDTA
hThrombin 2196-SE 42 μg/mL 1, 10-Phenanthroline
rmTryptase-5 2634-SE 0.2 μg/mL 1, 10-Phenanthroline
rmTryptase epsilon 2059-SE 0.3 μg/mL 1, 10-Phenanthroline
rhTryptase beta2 3796-SE 0.1 μg/mL 1, 10-Phenanthroline
rmFactor VII 3178-PA 1.0 μg/mL 1, 10-Phenanthroline
rhProprotein Convertase 7 2984-SE 2.0 μg/mL 1, 10-Phenanthroline
rm Prss28 4808-SE 0.1 μg/mL 1, 10-Phenanthroline
rhFactor XIV/Protein C 3349-SE 2.3 μg/mL 1, 10-Phenanthroline
rmFactor XIV/Protein C 4885-SE 5.0 μg/mL 1, 10-Phenanthroline

Additional Thermolysin Products

Product Documents for Bacterial Thermolysin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Bacterial Thermolysin Protein, CF

For research use only

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