Could you tell me biological activity of # NBC1-28779?

Name: Recombinant Human Aurora A His Protein
Brand: Novus Biologicals
SKU: NBC1-28779

Unfortunately, we don't know whether this protein has function, because the enzymatic activity has never been tested. Since NBC1-28779 is expressed in E. coli, it is unlikely that it has function. If wanting to have any Aurora A protein with function, please check this: Product Search as all of these were expressed in the insect cell (Sf9) expression system. Furthermore, the specific activities for phosphorylating of serines/threonines have been posted.

Recombinant Human Aurora A His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBC1-28779

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Aurora A Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-403 of Human Aurora A

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDRSKENCIS GPVKATAPVG GPKRVLVTQQ FPCQNPLPVN SGQAQRVLCP SNSSQRIPLQ AQKLVSSHKP VQNQKQKQLQ ATSVPHPVSR PLNNTQKSKQ PLPSAPENNP EEELASKQKN EESKKRQWAL EDFEIGRPLG KGKFGNVYLA REKQSKFILA LKVLFKAQLE KAGVEHQLRR EVEIQSHLRH PNILRLYGYF HDATRVYLIL EYAPLGTVYR ELQKLSKFDE QRTATYITEL ANALSYCHSK RVIHRDIKPE NLLLGSAGEL KIADFGWSVH APSSRRTTLC GTLDYLPPEM IEGRMHDEKV DLWSLGVLCY EFLVGKPPFE ANTYQETYKR ISRVEFTFPD FVTEGARDLI SRLLKHNPSQ RPMLREVLEH PWITANSSKP SNCQNKESAS KQS

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

47.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Aurora A His Protein

SDS-PAGE: Recombinant Human Aurora A His Protein [NBC1-28779]

SDS-Page: Recombinant Human Aurora A Protein [NBC1-28779] - Aurora kinase A, 47.9 kDa (423aa) with a purity of 95% by SDS - PAGE

Formulation, Preparation and Storage

NBC1-28779

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.5 mM DTT, 100 mM NaCl, 0.1 mM EDTA, 0.1 mM EGTA, 0.1 mM PMSF, 20% glycerol
Preservative	No Preservative
Concentration	0.5 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Aurora A

Aurora Kinase A is a member of a family of mitotic serine/threonine kinases. It is implicated with important processes during mitosis and meiosis whose proper function is integral for healthy cell proliferation. Aurora kinase A is highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also this protein plays a crucial role in tumourigenesis and is overexpressed in many kinds of cancers. Recombinant human Aurora-A fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Alternate Names

AIK, ARK1, AURA, AURKA, BTAK, STK15, STK6, STK7

Gene Symbol

AURKA

Additional Aurora A Products

Product Documents for Recombinant Human Aurora A His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human Aurora A His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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