Human Immunotherapy Luminex® Performance Assay 24-plex Fixed Panel

Name: Human Immunotherapy Luminex® Performance Assay 24-plex Fixed Panel
Brand: R&D Systems
SKU: LKTM010

Catalog # LKTM010 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

New Version Available - LKTM010B

Discontinued Product

LKTM010 has been discontinued and is replaced by LKTM010B.

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Magnetic bead-based multiplex assay for the Luminex^® platform
Format:	1 x 96-well microplate and magnetic antibody-coated beads
Species:	Human

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View Full Technical Details for Human Immunotherapy Luminex® Performance Assay 24-plex Fixed Panel

Technical Data
Citations (2) / Reviews

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human PD-L1/B7-H1 with up to -1 analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

	n=40 (20x2 levels)	n=25
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
B7-H1/PD-L1	1.8%	11.3%
CCL2/MCP-1	1.4%	8.9%
CCL3/MIP-1alpha	1.5%	7.3%
CCL4/MIP-1beta	3.0%	9.6%
CD40 Ligand	3.3%	10.6%
CXCL10/IP-10	2.1%	8.8%
CXCL8/IL-8	1.8%	9.0%
GM-CSF	3.1%	10.3%
Granzyme B	1.8%	11.0%
IFN-alpha	2.9%	9.9%
IFN-gamma	2.8%	10.4%
IL-10	3.3%	9.0%
IL-12 p70	2.3%	10.5%
IL-13	1.6%	9.7%
IL-15	2.4%	9.9%
IL-17A	2.5%	11.2%
IL-1alpha	3.0%	10.4%
IL-1beta	2.3%	10.0%
IL-1ra	2.1%	10.3%
IL-2	2.2%	9.2%
IL-33	2.4%	12.3%
IL-4	3.2%	9.7%
IL-6	2.6%	9.8%
TNF-alpha	2.2%	8.7%

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an astrisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
*Natural linearity
B7-H1/PD-L1	4	106%	4	109%
CCL2/MCP-1	4*	82%		High endogenous
CCL3/MIP-1alpha	4	77%	4	139%
CCL4/MIP-1beta	4	103%	4	111%
CD40 Ligand	4	113%	4	100%
CXCL10/IP-10	3	110%		High endogenous
CXCL8/IL-8	4	118%	4	96%
GM-CSF	4	96%	4	110%
Granzyme B	4	99%	4	118%
IFN-alpha	3	98%	4	101%
IFN-gamma	4	97%	4	112%
IL-10	4	111%	4	104%
IL-12 p70	4	106%	4	108%
IL-13	4	112%	4	103%
IL-15	3	88%	3	89%
IL-17A	4	110%	4	106%
IL-1alpha	4	143%	4	80%
IL-1beta	4	107%	4	99%
IL-1ra	4*	100%	4	145%
IL-2	4	105%	4	102%
IL-33	4	93%	4	110%
IL-4	3	127%	3	77%
IL-6	3	124%	3	90%
TNF-alpha	4	117%	4	69%

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Scientific Data Examples

	Human Immunotherapy Luminex Panel Standard Curves

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Specific Notices

Luminex® is a registered trademark of Luminex Corporation.

Citations for Human Immunotherapy Luminex® Performance Assay 24-plex Fixed Panel (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human Immunotherapy Luminex® Performance Assay 24-plex Fixed Panel include:

Species: Human
Sample Types: Cell Culture Supernatants

V Yekehfalla et al. (2022-09-14), Generation and Functional Characterization of PLAP CAR-T Cells against Cervical Cancer Cells Biomolecules, 2022-012(9).
PMID: 36139135
Species: Human
Sample Types: Plasma

M Vono et al. (2021-09-15), Robust innate responses to SARS-CoV-2 in children resolve faster than in adults without compromising adaptive immunity Cell Reports, 2021-00(0):109773.
PMID: 34587479

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