Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay

Name: Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay
Brand: R&D Systems
SKU: FCSTM14
Rating: 5 (2 reviews)

Catalog # FCSTM14 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (1)

Product Datasheet / COA / SDS

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Luminex High Performance Assays

Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.

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Key Product Details

Panel:	Luminex Performance Human High Sensitivity Cytokine Panel B
Assay Type:	Magnetic bead-based multiplex assay for the Luminex® MAGPIX® platform
Format:	96-well microplate and antibody-coated magnetic beads*
Species:	Human
Analytes Detected:	GM-CSF IFN-gamma IL-1 beta/IL-1F2 IL-2 IL-5 IL-6 IL-7 IL-13 IL-15 IL-17/IL-17A IL-17F IL-22 IL-23 IL-31 IL-33 IL-36 beta/IL-1F8 TNF-alpha

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services .

View Full Technical Details for Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay

Technical Data
Citations (1) / Reviews (2)

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile up to 17 analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Kit Components

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

	n=20 (10x2 levels)	n=43
Intra-Assay Precision - is generated from the mean of the %CV’s from 20 reportable results across two different concentrations of analytes in a single serum assay. Inter-Assay Precision - is generated from the mean of the %CV’s across two different concentrations of analytes across 43 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
GM-CSF	1.7	9.8
IFN-gamma	1.5	15.6
IL-1 beta	1.7	11.1
IL-2	2.0	11.4
IL-5	3.2	14.1
IL-6	2.0	11.4
IL-7	2.4	12.4
IL-10	2.2	12.8
IL-13	2.9	12.3
IL-15	2.0	12.7
IL-17A	1.6	11.7
IL-17F	2.5	9.9
IL-22	2.4	16.4
IL-23	1.1	15.0
IL-31	2.3	14.8
IL-33	1.9	13.3
IL-36 beta	2.5	13.0
TNF-alpha	1.7	11.6

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
GM-CSF	4	92	4	104
IFN-gamma	4	96	4	101
IL-1 beta	4	94	4	111
IL-2	4	96	4	98
IL-5	4	101	4	103
IL-6	4	107	4	83
IL-7	4	101	4	94
IL-10	4	106	4	104
IL-13	4	91	4	117
IL-15	4	94	4	110
IL-17A	4	112	4	100
IL-17F	4	118	4	75
IL-22	4	105	4	86
IL-23	4	94	4	78
IL-31	4	94	4	108
IL-33	4	90	4	125
IL-36 beta	4	101	4	105
TNF-alpha	4	107	4	94

Build Your Assay

Make Your Selections Below to Configure Your Custom Luminex Assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Scientific Data Examples

	Human Cytokine B High Sensitivity Luminex Panel Standard Curves

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Specific Notices

Luminex® is a registered trademark of Luminex Corporation.

Citations for Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human High Sensitivity Cytokine B Premixed Magnetic Luminex® Performance Assay include:

Species: Human
Sample Types: Cell Culture Supernates

A Morou et al. (2019-07-15), Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease Nat. Immunol., 2019-020(8):1059-1070.
PMID: 31308541

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