Key Product Details

Species Reactivity

Goat

Applications

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Goat IgG

Concentration

Please contact technical services for concentration.

View all IgG Isotype Controls »

Product Specifications

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for Goat IgG Isotype Control [DyLight 405]

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Fluorophore-linked immunosorbent assay

1:100 - 1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.

Recommended applications are based on validated applications from the unconjugated base product NB410-28088. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Spectra Viewer

Formulation, Preparation, and Storage

Formulation

50 mM Sodium Borate.

Preservative

0.05% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Gene Symbol

IGHG1

Additional IgG Products

Product Documents for Goat IgG Isotype Control [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Goat IgG Isotype Control [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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