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Fast-track your development timelinesiCE3's 10-minute start to finish runs with the HT Cartridge lets you optimize charge heterogeneity method conditions in an afternoon. You can also use the same platform method for multiple molecules. There is no need for product-specific methods. That means you can standardize platform methods across product development and QC, so you'll save time and costs and get robust and reproducible data. |
View all citations for the Imaged Capillary Electrophoresis (iCE) platform.
Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.
"For charge variant analysis, we have largely replaced ion exchange chromatography with the icIEF on the Maurice system….. Once we brought it in-house, it was the ideal platform for us - the icIEF fractionation capability enabled us to get further insights into the charge variants."
Arnaud Delobel, PhD, Director, R&D and Innovation Quality Assistance
"Imaged cIEF, also known as icIEF, is a platform analytical technology and offers not only high throughput with a quick turn-around of ~10-min per sample, but also quantitation capability for charge variants, while maintaining the highest resolution of IEF technology."
Xiaoping He, Sr. Principal Scientist, Pfizer (retired)
"Adding fractionation capabilities can really help in simplifying the workflow to assess peak identity, especially if paired with state-of-the-art mass spectrometry techniques. On top of this, the experience of working with the Bio-Techne family has been a pleasure so far and they helped us throughout our first work, beyond the training aspect of the installation."
Dr. Sara Carillo, Bioanalytical Research Lead, NIBRT
"Replacing preparative chromatography with MauriceFlex has made the process of fraction collection more efficient."
Amreen Jonas, Principal Associate Scientist, Mersana Therapeutics
"I think that the data we produced [on Maurice] shows excellent quality, due to the precise and reliable Maurice instrument."
Professor Hermann Wätzig, Technical University of Braunschweig in Germany
Protein samples are first premixed with carrier ampholytes, additives and pI markers. Samples are separated in a capillary cartridge with electrolytic tanks at each end—one tank is filled with acid (anolyte) and the other base (catholyte).
The sample mixture is injected to fill the entire capillary column. Voltage is then applied to the anolyte and catholyte tanks. This creates a pH gradient, which separates and focuses the proteins based on their pI. The whole-column UV detector monitors the entire process in the capillary in real time. The focusing time can be optimized in a single sample run, and once it's complete the separation pattern is captured and analyzed. The capillary column is then washed so it's ready to go for the next sample.
| Feature | Description |
|---|---|
| Sample Volume/Run | 12-17 µL |
| Sample Delivery | Alcott 720 Autosampler or PrinCE Next Microinjector |
| Typical Run Time | 10-15 minutes |
| Detection | UV absorption at 280 nm |
| Focusing Voltage | 600 V/cm |
| Dimensions | 66 cm H x 28 cm W x 31 cm D |
| Weight | 20 kg (45 lbs) |
| Power | 120-230 V AC, 50/60 Hz |
