Trypan Blue Solution 0.4%, sterile filtered

Name: Trypan Blue Solution 0.4%, sterile filtered
Brand: Tocris Bioscience
SKU: 8916

Tocris Bioscience, part of Bio-Techne | Catalog # 8916

COA / SDS

A common dye enabling accurate assessment of cell viability by selectively staining dead cells, for cell therapy applications

Key Product Details

Description:	A common dye enabling accurate assessment of cell viability by selectively staining dead cells, for cell therapy applications
Chemical Name:	Sodium 3,3'-((1E,1'E)-(3,3'-Dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(5-amino-4-hydroxynaphthalene-2,7-disulfonate)
Molecular Weight:	960.79

View Full Technical Details for Trypan Blue Solution 0.4%, sterile filtered

Technical Data
Citations

Biological Activity for Trypan Blue Solution 0.4%, sterile filtered

Trypan Blue Solution 0.4%, sterile filtered is a common dye enabling accurate assessment of cell viability. It can be used in a dye exclusion test to determine the number of live and dead cells in a sample. This test is based on the principle that Trypan Blue only enters cells with damaged membranes, making them appear blue and leaving live cells unstained. Trypan Blue Solution 0.4%, sterile filtered can be used for cell therapy applications.

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Technical Data for Trypan Blue Solution 0.4%, sterile filtered

M. Wt	960.79
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄
Storage	Store at RT
CAS Number	72-57-1
PubChem ID	137122314
InChi Key	GLNADSQYFUSGOU-GPTZEZBUSA-J
Smiles	CC1=CC(C2=CC(C)=C(/N=N/C3=C(O)C(C(N)=CC(S(=O)(O[Na])=O)=C4)=C4C=C3S(=O)(O[Na])=O)C=C2)=CC=C1/N=N/C5=C(O)C6=C(C=C5S(=O)(O[Na])=O)C=C(S(=O)(O[Na])=O)C=C6N

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Trypan Blue Solution 0.4%, sterile filtered

Solubility

Soluble in PBS (Supplied pre-dissolved at a concentration of 0.4%)

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References for Trypan Blue Solution 0.4%, sterile filtered

References are publications that support the biological activity of the product.

Pourzia Quantifying requirements for mitochondrial apoptosis in CAR T killing of cancer cells. Cell Death Dis. 2023 PMID: 37055388
Liu Challenges of cell counting in cell therapy products. Cell Transplant. 2024 PMID: 39462979