TFAX 488, TFP
Tocris Bioscience, part of Bio-Techne | Catalog # 6621
Green fluorescent dye; amine reactive
Discontinued Product
6621 has been discontinued.
View all TFAX Dyes products.
Key Product Details
Description
Green fluorescent dye; amine reactive
Product Description
TFAX 488, TFP is an amine reactive green fluorescent dye. Insensitive to pH in the range 4 - 10. Forms bright and photostable conjugates with proteins and antibodies. Less susceptible to spontaneous hydrolysis during conjugation reactions compared to SE or NHS dyes. Also displays greater stability compared to NHS and SE versions, typically lasting several hours. Suitable for use in flow cytometry, two-photon excitation microscopy (TPE), and super resolution microscopy techniques, such as dSTORM, SIM and STED. Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1cm-1; quantum yield = 0.92.Optical Data
| Emission Color | Green |
| Brightness | |
| λabs | 495 nm |
| λem | 515 nm |
| Extinction Coefficient (ε) | 73,000 M-1cm-1 |
| Quantum Yield (φ) | 0.92 |
| Closest Laser line | 488 nm |
| Reactive Group | TFP |
| Reactivity | Primary amines |
| Cell Permeable | No |
| pH Sensitivity | pH insensitive pH 4-10 |
| Application | flow cytometry, two-photon excitation microscopy, dSTORM, SIM, STED |
| Alternative For | Alexa Fluor® 488 TFP ester, AF 488, FITC, Cy2 |
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Product Specifications
Molecular Weight
884.91
Formula
C27H14F4N2O11S2.C12H30N2
Storage
Store at -20°C
Chemical Name
3,6-Diamino-9-[2-carboxy-4-[(2,3,5,6-tetrafluorophenoxy)carbonyl]phenyl]-4,5-disulfoxanthylium bis(triethylammmonium) salt
CAS Number
878546-79-3
SMILES
CCN(CC)CC.CCN(CC)CC.[NH2+]=C1C=CC2=C(C3=CC=C(C(OC4=C(F)C(F)=CC(F)=C4F)=O)C=C3C([O-])=O)C5=CC=C(N)C(S(=O)(O)=O)=C5OC2=C1S(=O)(O)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Schilling On the seeding and oligomerization of pGlu-amyloid peptides (in vitro). Biochemistry 2006 PMID: 17029395
Product Specific Notices for TFAX 488, TFP
For research use only
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