Mag-Fura-2 AM

Name: Mag-Fura-2 AM
Brand: Tocris Bioscience
SKU: 7855

Tocris Bioscience, part of Bio-Techne | Catalog # 7855

COA / SDS

Magnesium (Mg2+) indicator, membrane permeable

Key Product Details

Description:	Magnesium (Mg²⁺) indicator, membrane permeable
Chemical Name:	Bis(acetoxymethyl) 2,2'-((5-(2-(acetoxymethoxy)-2-oxoethoxy)-2-(5-((acetoxymethoxy)carbonyl)oxazol-2-yl)benzofuran-6-yl)azanediyl)diacetate
Purity:	≥95% (HPLC)
Molecular Weight:	722.57

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Technical Data
Citations

Biological Activity for Mag-Fura-2 AM

Mag-Fura-2 AM is a cell-permeable fluorescent magnesium ion (Mg²⁺) indicator. It shows selectivity for Mg²⁺ over other bivalent ions (Zn²⁺, Cu²⁺ and Ca²⁺). It exhibits >13,000-fold selectivity for Mg²⁺ ions over Ca²⁺ions (Mg²⁺ Kd = 1.9 nM vs Ca²⁺ Kd = 25 μM). Excitation and emission maxima (λ) are 369 and 511 nm, respectively. Mag-Fura-2 AM can be used as an indirect indicator of ATP consumption/production.

It is recommended to prepare stock solutions in DMSO.

Optical Data for Mag-Fura-2 AM

λ_abs	369 nm
λ_em	511 nm
Extinction Coefficient (ε)	31000 M^-1cm^-1
Cell Permeable	Yes
Application	Confocal Imaging, Flow Cytometry

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Technical Data for Mag-Fura-2 AM

M. Wt	722.57
Formula	C₃₀H₃₀N₂O₁₉
Storage	Store at -20°C
Purity	≥95% (HPLC)
CAS Number	130100-20-8
PubChem ID	131176
InChi Key	PCDUVTXECKTHBH-UHFFFAOYSA-N
Smiles	O=C(C1=CN=C(C2=CC3=C(C=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCC(OCOC(C)=O)=O)=C3)O2)O1)OCOC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

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References for Mag-Fura-2 AM

References are publications that support the biological activity of the product.

Watanabe Chronic magnesium deficiency causes reversible mitochondrial permeability transition pore opening and impairs hypoxia tolerance in the rat heart. J.Pharmacol.Sci. 2022 PMID: 35063139
Cheng Variability of mitochondrial energy balance across brain regions. J.Neurochem. 2021 PMID: 33190229
Schütz Trophectoderm cell failure leads to peri-implantation lethality in Trpm7-deficient mouse embryos. Cell Rep. 2021 PMID: 34686339