Janelia Fluor® 635, Haloalkane
Tocris Bioscience, part of Bio-Techne | Catalog # 8808
Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag (SLT) substrate
Key Product Details
Description
Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag (SLT) substrate
Product Description
Key Information: Janelia Fluor® 635, Haloalkane is a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 635, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Turn-on ratio in vitro A/A0 = 113, F/F0 = 67. Exhibits low non-specific background staining.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| Brightness | |
| λabs | 635 nm |
| λem | 652 nm |
| Extinction Coefficient (ε) | 167000 M-1cm-1 |
| Quantum Yield (φ) | 0.56 |
| Closest Laser line | 635 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
Spectra Viewer
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Product Specifications
Molecular Weight
738.34
Formula
C39H46ClF2N3O5Si
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate
CAS Number
1811539-66-8
PubChem ID
140879857
InChI Key
SSLCQPFUOBJTQH-UHFFFAOYSA-N
SMILES
O=C(NCCOCCOCCCCCCCl)C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CC(F)C3)C=C2[Si]4(C)C)=C(C=C/5)C4=CC5=[N+]6CC(F)C\6)=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.38 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 635, Haloalkane
The following data is based on the product molecular weight 738.34
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.54 mL | 67.72 mL | 135.44 mL |
| 0.5 mM | 2.71 mL | 13.54 mL | 27.09 mL |
| 1 mM | 1.35 mL | 6.77 mL | 13.54 mL |
| 5 mM | 0.27 mL | 1.35 mL | 2.71 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Cook HaloTag-based reporters for fluorescence imaging and biosensing. Chembiochem. 2023 PMID: 36815462
- Tsai Nuclear microenvironments modulate transcription from low-affinity enhancers. Elife 2017 PMID: 29095143
- Grimm A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat. Methods 2017 PMID: 28869757
Product Specific Notices for Janelia Fluor® 635, Haloalkane
For research use only
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