Janelia Fluor® 635, Haloalkane
Tocris Bioscience, part of Bio-Techne | Catalog # 8808
Key Product Details
| Description: | Cell-permeable fluorogenic fluorescent dye with a chloroalkane handle. Application: live-cell imaging as a self-labeling tag (SLT) substrate |
| Chemical Name: | 4-((2-(2-((6-Chlorohexyl)oxy)ethoxy)ethyl)carbamoyl)-2-(3-(3-fluoroazetidin-1-ium-1-ylidene)-7-(3-fluoroazetidin-1-yl)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate |
| Purity: | ≥95% (HPLC) |
| Molecular Weight: | 738.34 |
Biological Activity for Janelia Fluor® 635, Haloalkane
Key Information: Janelia Fluor® 635, Haloalkane is a red cell-permeable fluorogenic fluorescent dye with a chloroalkane handle.
Application: Live-cell imaging as a self-labeling tag substrate. Suitable for confocal microscopy, light sheet microscropy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Stable to fixation. Cell permeable.
Properties and Photophysical Data: Janelia Fluor® 635, Haloalkane shows a high degree of fluorogenicity. Excitation and emission maxima (λ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Turn-on ratio in vitro A/A0 = 113, F/F0 = 67. Exhibits low non-specific background staining.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
Optical Data for Janelia Fluor® 635, Haloalkane
| Emission Color | Red |
|---|---|
| Brightness | |
| λabs | 635 nm |
| λem | 652 nm |
| Extinction Coefficient (ε) | 167000 M-1cm-1 |
| Quantum Yield (φ) | 0.56 |
| Closest Laser line | 635 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy (SRM) |
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