HB-2-30
Tocris Bioscience, part of Bio-Techne | Catalog # 8831
Key Product Details
| Description: | Fluorescent TG2/LRP1 substrate for imaging endocytosis |
| Purity: | ≥90% (HPLC) |
| Molecular Weight: | 1307.59 |
Biological Activity for HB-2-30
Key information: HB-2-30 is a far-red fluorescent transglutaminase-2 (TG2) probe for imaging endocytosis in vitro and in vivo. HB-2-30 undergoes highly efficient endocytosis in complex with TG2 and α2-macroglobulin via the LRP1 pathway.
Used for: Imaging endocytosis in vitro and in vivo, explore targeted lysosomal degradation of secreted and cell surface proteins.
Application: Confocal microscopy.
Properties and Photophysical Data: Suitable for multiplexing and can be combined with DAPI (Cat. No. 5748) counter-staining. Excitation and emission maxima (λ) are 649 and 665 nm, respectively.
Licensing Information
Sold under license from Stanford University
Optical Data for HB-2-30
| λabs | 649 nm |
|---|---|
| λem | 665 nm |
| Application | Confocal microscopy |
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Application of HB-2-30 in vitro. Confocal microscopy images of bone marrow-derived dendritic cells from WT C57BL/6 mice (left) and TG2KO mice (right) incubated with 3μM HB-2-30. Cells were incubated for 90 minutes with the probe before being fixed, stained for nuclei (blue), and imaged. HB-2-30 signals is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford. |
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Application of HB-2-30 in vivo. Ileum tissue sections from mice dosed with 10 mg/kg HB-2-30. WT C57BL/6 mice (left) and TG2KO mice (right) were dosed with HB-2-30 and sacrificed after 2 hours. Intestines were Swiss rolled, thin sectioned, stained for nuclei (blue), and imaged via confocal fluorescence microscopy. HB-2-30 signal is in red. Image kindly provided by Fu-Chen Yang and Harrison Besser, University or Stanford. |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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