dTAG Janelia Fluor® 525
Tocris Bioscience, part of Bio-Techne | Catalog # 8102
Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Key Product Details
Description
Fluorogenic srTAG probe for live cell imaging of FBKP12 (F36V/L) fusion proteins
Product Description
Key Information: dTAG Janelia Fluor® 525 is a fluorogenic srTAG probe for live cell imaging of FKBP12F36V/L fusion proteins.Application: Suitable for confocal microscopy and Super Resolution Microscopy (SRM) including STED. dTAG Janelia Fluor® 525 is cell permeable.
Properties and Photophysical Data: In FRAP (fluorescence recovery after photobleaching) experiment, the highest maximum recovery ratio (73%) was shown when U2OS cells expressing FKBPF36L were incubated with 10 μM dTAG Janelia Fluor® 525; excitation and emission maxima (λ) are 530 nm and 560 nm, respectively; quantum yield = 0.47; extinction coefficient = 82,800 M-1cm-1 (in 4% TFA).
It is recommended to use a working concentration of 10 μM in live cell imaging application.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Optical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 530 nm |
| λem | 560 nm |
| Extinction Coefficient (ε) | 82800 M-1cm-1 |
| Quantum Yield (φ) | 0.47 |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Confocal Microscopy, Super Resolution Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
1244.30
Formula
C67H69F4N5O14
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
2-(3-(3,3-Difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)-4-((2-(2-(3-((R)-3-(3,4-dimethoxyphenyl)-1-(((S)-1-((S)-2-(3,4,5-trimethoxyphenyl)butanoyl)piperidine-2-carbonyl)oxy)propyl)phenoxy)acetamido)ethyl)carbamoyl)benzoate
InChI Key
ZSEJCFOGOGMEPJ-FUJQAITOSA-N
SMILES
O=C([C@@H]1CCCCN1C([C@H](C2=CC(OC)=C(OC)C(OC)=C2)CC)=O)O[C@@H](C3=CC(OCC(NCCNC(C4=CC=C(C([O-])=O)C(C(C5=CC=C(N6CC(F)(F)C6)C=C5O7)=C(C=C/8)C7=CC8=[N+]9CC(F)(F)C\9)=C4)=O)=O)=CC=C3)CCC%10=CC(OC)=C(OC)C=C%10
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.44 | 10 |
Preparing Stock Solutions
for dTAG Janelia Fluor® 525
The following data is based on the product molecular weight 1244.30
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 8.04 mL | 40.18 mL | 80.37 mL |
| 0.5 mM | 1.61 mL | 8.04 mL | 16.07 mL |
| 1 mM | 0.80 mL | 4.02 mL | 8.04 mL |
| 5 mM | 0.16 mL | 0.80 mL | 1.61 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Du Self-renewable tag for photostable fluorescence imaging of proteins. J.Am.Chem.Soc. 2023 PMID: 37596976
Product Specific Notices for dTAG Janelia Fluor® 525
For research use only
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