BDY FL Staurosporine

Product Description

BDY FL Staurosporine is a fluorescent kinase-targeting probe. It can be paired with antibodies labelled with TR-FRET donors such as CoraFluor™ 1 (Cat. No. 7920) for high throughput binding and protein quantification assays. BDY FL Staurosporine shows high specificity for tyrosine kinase (TK) and tyrosine kinase-like (TKL) family kinases. Excitation and emission maxima (λ) are 514 and 516 nm, respectively; quantum yield = 0.11; extinction coefficient = 35,000 M^-1cm^-1. The measured K_D values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file (see below).

Supplementary Information

The measured K_D values, from TR-FRET based saturation binding assay data, are provided in the accompanying supplementary file.

Optical Data

Solubility

Preparing Stock Solutions for BDY FL Staurosporine

The following data is based on the product molecular weight 901.82

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	11.09 mL	55.44 mL	110.89 mL
0.5 mM	2.22 mL	11.09 mL	22.18 mL
1 mM	1.11 mL	5.54 mL	11.09 mL
5 mM	0.22 mL	1.11 mL	2.22 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Hirozane Structure-based rational design of staurosporine-based fluorescent probe with broad-ranging kinase affinity for kinase panel application. Bioorg.Med.Chem.Lett. 2019 PMID: 31526603