7-Aminoactinomycin D
Tocris Bioscience, part of Bio-Techne | Catalog # 7121
Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy
Discontinued Product
7121 has been discontinued.
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Key Product Details
Description
Red-fluorescent DNA stain, membrane impermeant to live cells. Used for: apoptosis detection, cell viability staining. Application: flow cytometry, fluorescence microscopy
Product Description
Key information: 7-aminoactinomycin D (7-AAD) is a red-fluorescent DNA stain. It is membrane impermeant to live cells but penetrates dead or damaged cells.Used for: cell cycle studies, selective detection of dead cells by flow cytometry, chromosome banding analysis, cell sorting, and apoptosis quantification.
Application: fluorescence microscopy and flow cytometry.
Properties and Photophysical Data: G-C base-specific DNA intercalator that forms stable complexes with DNA. May be used in combination with FITC (Cat. No. 5440) or phycoerythrin (PE). Fluorescent analogue of actinomycin D (Cat. No. 1229). Can be excited using 488 nm (blue), 532 nm (green), and 561 nm (yellow-green) laser lines. Suitable for multicolor fluorescence microscopy experiments. Excitation and emission maxima (λ) are 549 nm and 655 nm, respectively.
Optical Data
| λabs | 549 nm |
| λem | 655 nm |
| Application | Fluorescence Microscopy, Flow Cytometry |
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Product Specifications
Molecular Weight
1270.45
Formula
C62H87N13O16
Storage
Store at -20°C
Purity
≥97% (HPLC)
Chemical Name
2,7-Diamino-N1,N9-bis(6,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentaoxohexadecahydro-1H-pyrrolo[2,1-i][1]oxa[4,7,10,13]tetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-oxo-3H-phenoxazine-1,9-dicarboxamide
CAS Number
7240-37-1
PubChem ID
14924508
InChI Key
YXHLJMWYDTXDHS-UHFFFAOYSA-N
SMILES
NC1=C(C(NC(C(C)OC(C(C(C)C)N(C(CN(C)C2=O)=O)C)=O)C(NC(C(C)C)C(N3C2CCC3)=O)=O)=O)C4=NC5=C(C(NC(C(NC(C(C)C)C6=O)=O)C(C)OC(C(C(C)C)N(C(CN(C)C(C7N6CCC7)=O)=O)C)=O)=O)C=C(N)C(C)=C5OC4=C(C)C1=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2 |
Preparing Stock Solutions
for 7-Aminoactinomycin D
The following data is based on the product molecular weight 1270.45
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.02 mM | 39.36 mL | 196.78 mL | 393.56 mL |
| 0.1 mM | 7.87 mL | 39.36 mL | 78.71 mL |
| 0.2 mM | 3.94 mL | 19.68 mL | 39.36 mL |
| 1 mM | 0.79 mL | 3.94 mL | 7.87 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Schmid Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry. Cytometry 1992 PMID: 1547670
- Philpott The use of 7-amino actinomycin D in identifying apoptosis: Simplicity of use and broad spectrum of application compared with other techniques. Blood 1996 PMID: 8630384
- Zelenin 7-Amino-actinomycin D as a specific fluorophore for DNA content analysis by laser flow cytometry. Cytometry 1984 PMID: 6468175
Product Specific Notices for 7-Aminoactinomycin D
For research use only
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