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Rat Wnt-10b ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00485

Novus Biologicals, part of Bio-Techne
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NBP3-00485

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Wnt-10b in samples. No significant cross-reactivity or interference between Human Wnt-10b and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) %CV < 4.7

Inter-Assay Precision (Precision between assays) %CV < 4.82

Recovery for Rat Wnt-10b ELISA Kit (Colorimetric)

Recovery

86 - 104%

Linearity

Rat Wnt-10b ELISA Kit (Colorimetric)

Scientific Data Images for Rat Wnt-10b ELISA Kit (Colorimetric)

Rat Wnt-10b ELISA Kit (Colorimetric)

ELISA: Rat Wnt-10b ELISA Kit (Colorimetric) [NBP3-00485] -

ELISA: Rat Wnt-10b ELISA Kit (Colorimetric) [NBP3-00485] - Standard Curve Reference

Kit Contents for Rat Wnt-10b ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Wnt-10b

Researchers have indicated that they want access to novel target antibodies quickly to aid in their research. Novus is pleased to offer researchers access to products that are not fully characterized or validated. This antibody is shown by Peptide ELISA to bind to the peptide used as immunogen. Investigators should empirically determine the suitability of the antibody, including optimal dilutions, for other applications of interest. * We cannot guarantee that this antibody will work in any application other than in a Peptide ELISA against the peptide used as immunogen and therefore can not offer a refund if the antibody does not work in your application. * The antibody is offered at a lower price compared to more highly validated antibodies. * We are not able to provide the peptide used as immunogen for this product. As this antibody is not fully validated we appreciate your feedback. Customer feedback is used to help generate testing methodologies which can lead to further product validation or withdrawal.__________________________ Wnts comprise a family of secreted signaling proteins that regulate diverse developmental processes. Activation of Wnt signaling by Wnt10b inhibits differentiation of preadipocytes and blocks adipose tissue development. In bone development, Wnt10b shifts cell fate toward the osteoblast lineage.

Long Name

Wingless-type MMTV Integration Site Family, Member 10B

Alternate Names

Wnt-12, Wnt10b

Gene Symbol

WNT10B

Additional Wnt-10b Products

Product Documents for Rat Wnt-10b ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Wnt-10b ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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