Rabbit Procollagen Type 1 N-Terminal Propeptide ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42381

Novus Biologicals, part of Bio-Techne
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NBP3-42381
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids (50 uL)

Sensitivity

90.4 pg/mL

Assay Range

246.9 - 20000 pg/mL

View all Procollagen Type 1 N-Terminal Propeptide ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rabbit

Description

Assay time: 2 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10

Inter-Assay Precision (Precision between assays) %CV < 12

Recovery

Recovery

78 - 99%

Kit Contents

  • Assay Diluent A
  • Assay Diluent B
  • Detection Reagent A
  • Detection Reagent B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated ready to use 96-well strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Procollagen Type 1 N-Terminal Propeptide

Procollagen type I N-terminal propeptide, also called P1NP, is a bone formation marker that is indicative of type I collagen disposition (1). P1NP is generated from the major bone protein Type I Collagen and is cleaved by proteases at the amino (N) terminal and released into the bloodstream (1-3). Typically, P1NP is released in a trimeric form, consisting of two alpha-1 chains and one alpha-2 chain, prior to being broken-down into monomers or fragments (1). Antibodies to P1NP commonly detect the trimeric structure using ELISA or radioimmunoassay applications (1). P1NP is most notably known for being a bone turnover marker, along with CTX-1, and is particularly useful for osteoporosis studies (2-4). While P1NP is used to detect bone formation, C-terminal telopeptide of type I collagen (CTX-1) is contrastingly used for bone resorption (2-4). Bone mineral density (BMD) as measured by dual-energy X-ray absorptiometry (DXA) is a common measurement of osteoporosis status and is frequently used in conjunction with P1NP and CTX-1 levels (2-4). P1NP can be collected and measured either via a blood test or urine test and is beneficial in that its levels are largely unaffected by food intake or circadian rhythms (3-4). One study of postmenopausal females found that the osteoporotic group had significantly higher average P1NP levels (51.7 ng/mL), suggestive of high bone turnover, compared to the normal range in the healthy, control group (38.9 ng/mL) and is inversely correlated with BMD (2). P1NP levels have also been shown to increase following antiresorptive therapy (3). Additionally, P1NP is considered a diagnostic marker for bone metastasis in breast cancer and prostate cancer (5). Specifically, studies found elevated P1NP serum levels is correlated with presence of bone metastasis and skeletal lesions (5).

References

1. Kuo, T. R., & Chen, C. H. (2017). Bone biomarker for the clinical assessment of osteoporosis: recent developments and future perspectives. Biomarker Research. https://doi.org/10.1186/s40364-017-0097-4

2. Kucukalic-Selimovic, E., Valjevac, A., & Hadzovic-Dzuvo, A. (2013). The utility of procollagen type 1 N-terminal propeptide for the bone status assessment in postmenopausal women. Bosnian Journal of Basic Medical Sciences. https://doi.org/10.17305/bjbms.2013.2337

3. Wheater, G., Elshahaly, M., Tuck, S. P., Datta, H. K., & van Laar, J. M. (2013). The clinical utility of bone marker measurements in osteoporosis. Journal of Translational Medicine. https://doi.org/10.1186/1479-5876-11-201

4. Williams, C., & Sapra, A. (2020). Osteoporosis Markers. In StatPearls. StatPearls Publishing.

5. D'Oronzo, S., Brown, J., & Coleman, R. (2017). The role of biomarkers in the management of bone-homing malignancies. Journal of Bone Oncology. https://doi.org/10.1016/j.jbo.2017.09.001

Alternate Names

Alpha-1 type I collagen, alpha1(I) procollagen, amino-terminal propeptide, CAFYD, COL1A1, collagen alpha 1 chain type I, collagen alpha-1(I) chain, collagen alpha-1(I) chain preproprotein, collagen of skin, tendon and bone, alpha-1 chain, collagen, type I, alpha 1, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, PINP, pro-alpha-1 collagen type 1, type I proalpha 1, Type I Procollagen Alpha 1 Chain

Additional Procollagen Type 1 N-Terminal Propeptide Products

Product Documents

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Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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