Mouse/Rat PDGF-AB DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY8460-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
7.81-500 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse, Rat
Mouse/Rat PDGF-AB DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse/Rat PDGF-AB DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse and rat Platelet-Derived Growth Factor AB (PDGF-AB). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse/Rat PDGF-AB DuoSet ELISA
Mouse/Rat PDGF-AB ELISA Standard Curve
Kit Contents for Mouse/Rat PDGF-AB DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
Normal Goat Serum: (Catalog # DY005)
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AB
PDGF-A and PDGF-B are platelet-derived growth factors that can form disulfide-linked homodimers or heterodimers with each other. These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of PDGF R alpha and R beta. PDGF-AB binds to alpha receptor homodimers as well as the heterodimeric receptor. PDGF-AA and -AB promote cell proliferation, cell survival, cytoskeletal rearrangement, cell migration and extracellular matrix production. They are important for the development of oligodendrocytes, testicular Leydig cells, alveolar smooth muscle cells, hair follicles, and intestinal villus.
Long Name
Platelet-derived Growth Factor AB
Alternate Names
PDGFAB
Additional PDGF-AB Products
Product Documents for Mouse/Rat PDGF-AB DuoSet ELISA
Product Specific Notices for Mouse/Rat PDGF-AB DuoSet ELISA
For research use only
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