Mouse Oncostatin M (OSM) Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MSM00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
4.7 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse Oncostatin M (OSM) Quantikine ELISA Kit
The Quantikine Mouse Oncostatin M (OSM) Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse OSM levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse OSM and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse OSM accurately. Results obtained using natural mouse OSM showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse OSM.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse OSM.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 36.7 | 62.1 | 127 | 36.5 | 65.3 | 135 |
| Standard Deviation | 2.36 | 1.32 | 3.64 | 4.53 | 6.20 | 9.20 |
| CV% | 6.4 | 2.1 | 2.9 | 12.4 | 9.5 | 6.8 |
Recovery for Mouse Oncostatin M (OSM) Quantikine ELISA Kit
The recovery of mouse OSM spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 92-103 |
| EDTA Plasma (n=4) | 95 | 92-100 |
| Heparin Plasma (n=4) | 99 | 94-106 |
| Serum (n=4) | 100 | 95-109 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse OSM were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse Oncostatin M (OSM) Quantikine ELISA Kit
Mouse Oncostatin M/OSM ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Oncostatin M/OSM
Oncostatin M (OSM) is a member of the IL-6 family of cytokines which share gp130 as a receptor subunit. the OSM-gp130 complex can recruit either LIF R alpha or OSM R beta, enabling OSM to act inflammation, neuroprotection, hematopoiesis, metabolism. and development. It also plays a role in tumor cell growth and antiviral immunity.
Alternate Names
MGC20461, oncostatin M, oncostatin-M
Gene Symbol
OSM
Additional Oncostatin M/OSM Products
Product Documents for Mouse Oncostatin M (OSM) Quantikine ELISA Kit
Product Specific Notices for Mouse Oncostatin M (OSM) Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.