Mouse MSP/MST1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MMSP0
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
Sensitivity
0.019 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Mouse MSP/MST1 Quantikine ELISA Kit
The Quantikine Mouse MSP/MST1 Immunoassay is a 4.5 hour solid phase ELISA
designed to measure mouse MSP in cell culture supernates, tissue lysates,
serum, plasma, and urine. It contains CHO cell-expressed recombinant mouse MSP
and antibodies raised against the recombinant factor. This immunoassay
has been shown to accurately quantitate recombinant mouse MSP.
Results obtained using natural mouse MSP showed linear curves that
were parallel to the standard curves obtained using the Quantikine kit
standards. These results indicate that this kit can be used to
determine relative mass values for natural mouse MSP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse MSP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.504 | 1.28 | 3.98 | 0.470 | 1.24 | 3.74 |
| Standard Deviation | 0.027 | 0.068 | 0.154 | 0.015 | 0.044 | 0.228 |
| CV% | 5.4 | 5.3 | 3.9 | 3.2 | 3.6 | 6.1 |
Recovery for Mouse MSP/MST1 Quantikine ELISA Kit
The recovery of mouse MSP spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 98 | 90-108 |
| Tissue Homogenates (n=4) | 101 | 90-109 |
| Urine (n=4) | 96 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MSP were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Background: MSP/MST1
Macrophage-stimulating protein (MSP), also known as Hepatocyte Growth Factor-like Protein (HGFL) and Scatter Factor-2 (SF2), is a member of the HGF family of growth factors. MSP is secreted as a single chain inactive precursor (pro-MSP). In response to injury, pro-MSP is activated by proteolytic cleavage. Activation results in the creation of alpha and beta chains joined by a disulfide bond.
Long Name
Macrophage Stimulating Protein/Macrophage Stimulating 1 [Hepatocyte Growth Factor-like]
Alternate Names
HGFL, MST1, SF2
Gene Symbol
MST1
Additional MSP/MST1 Products
Product Documents for Mouse MSP/MST1 Quantikine ELISA Kit
Product Specific Notices for Mouse MSP/MST1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.