Mouse IL-17D DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY2274
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
93.8-6000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse IL-17D DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Mouse IL-17D DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse IL-17D. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse IL-17D DuoSet ELISA
Mouse IL-17D ELISA Standard Curve
Kit Contents for Mouse IL-17D DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17D
Interleukin-17B is a disulfide-linked pro-inflammatory cytokine that functions in tumor and virus surveillance. IL-17 family cytokines signal through IL-17 RA, -B, -C, -D, -E, -H1, and -L. IL-17D is expressed preferentially in skeletal muscle, heart, adipose tissue, lung, pancreas, and the nervous system. It modulates immune responses indirectly by stimulating the production of myeloid growth factors and chemokines including IL-6, IL-8, and GM-CSF. In addition, IL-17D can suppress the proliferation of myeloid progenitors in colony formation assays.
Long Name
Interleukin 17D
Alternate Names
IL17D
Gene Symbol
IL17D
Additional IL-17D Products
Product Documents for Mouse IL-17D DuoSet ELISA
Product Specific Notices for Mouse IL-17D DuoSet ELISA
For research use only
Loading...
Loading...
Loading...