Mouse BCMA/TNFRSF17 DuoSet ELISA

Name: Mouse BCMA/TNFRSF17 DuoSet ELISA
Brand: R&D Systems
SKU: DY593

Catalog # DY593 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	78.125 - 5,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Mouse BCMA/TNFRSF17 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (1) / Reviews

Summary for Mouse BCMA/TNFRSF17 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse BCMA / TNFRSF17. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Mouse BCMA/TNFRSF17 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Mouse
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Mouse BCMA/TNFRSF17 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Mouse BCMA/TNFRSF17 DuoSet ELISA

	Mouse BCMA / TNFRSF17 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BCMA/TNFRSF17

B cell maturation protein (BCMA) is a member of the TNF receptor superfamily (TNFRSF17). BCMA is expressed in immune organs and mature Bcell lines. Although some expression has been observed at the cell surface, BCMA appears to be localized to the Golgi compartment. BCMA binding to APRIL or BAFF stimulates IgM production in peripheral blood B cells and increases the survival of cultured B cells. Mature human and mouse BCMA share 86% amino acid sequence identity.

Item	Value
Long Name	B Cell Maturation Factor
Entrez Gene IDs	608 (Human); 21935 (Mouse); 287034 (Rat); 102145399 (Cynomolgus Monkey)
Alternate Names	B cell maturation antigen, B-cell maturation protein, BCMA, BCMAtumor necrosis factor receptor superfamily member 17, BCMB-cell maturation factor, CD269, CD269 antigen, TNFRSF13A, TNFRSF17, tumor necrosis factor receptor superfamily, member 17

Citations for Mouse BCMA/TNFRSF17 DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse BCMA/TNFRSF17 DuoSet ELISA include:

Species: Mouse
Sample Types: Serum

X Ma et al. (2019-03-29), A novel xenograft mouse model for testing approaches targeting human kappa light-chain diseases Gene Ther., 2019-00(0).
PMID: 30926963

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Product Documents for Mouse BCMA/TNFRSF17 DuoSet ELISA

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