Human Total MMP-7 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DMP700
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (25 µL), Heparin Plasma (25 µL), Saliva (25 µL), Urine (50 µL)
Sensitivity
0.094 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine)
Product Summary for Human Total MMP-7 Quantikine ELISA Kit
The Quantikine Human MMP-7 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MMP-7 in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human MMP-7 and antibodies raised against the recombinant factor. The Quantikine kit detected both pro- and active forms of recombinant human MMP-7. Natural human MMP-7 showed dose-response curves that were parallel to the standard curves obtained using the kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-7.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human MMP-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Heparin Plasma, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.26 | 4.58 | 6.29 | 1.23 | 4.82 | 6.76 |
| Standard Deviation | 0.064 | 0.168 | 0.217 | 0.054 | 0.198 | 0.309 |
| CV% | 5.1 | 3.7 | 3.4 | 4.4 | 4.1 | 4.6 |
Recovery for Human Total MMP-7 Quantikine ELISA Kit
The recovery of MMP-7 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 96-108 |
| Urine (n=2) | 99 | 89-109 |
Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of MMP-7 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Scientific Data Images for Human Total MMP-7 Quantikine ELISA Kit
Human Total MMP-7 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-7
Matrix metalloproteinase 7, also known as Matrilysin, is a zinc and calcium dependent endopeptidase that is expressed in epithelial cells of normal and diseased tissues. It can digest a wide range of extracellular matrix proteins including Collagens IV and X, Gelatin, Casein, Laminin, Aggrecan, Entactin, Elastin, and Versican. MMP-7 can activate other proteinases such as Plasminogen, MMP-1, MMP-2, and MMP-9. In addition, MMP-7 regulates the activation or shedding of alpha-Defensin, TNF-alpha, and Fas Ligand.
Long Name
Matrix Metalloproteinase 7
Alternate Names
Matrilysin, MMP7
Gene Symbol
MMP7
Additional MMP-7 Products
Product Documents for Human Total MMP-7 Quantikine ELISA Kit
Product Specific Notices for Human Total MMP-7 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.