Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
R&D Systems, part of Bio-Techne | Catalog # DYC1770-2
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Sample Type
Cell Lysates
Reactivity
Human
Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human IGF-I R in cell lysates. An immobilized capture antibody specific for IGF-I R binds both phosphorylated and unphosphorylated IGF-I R . After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
Cell lysates (100 µL)
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Kit Contents for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I R/IGF1R
IGF-I R/IGF1R (Insulin-like Growth Factor I Receptor), also known as CD221, is a ubiquitously expressed heterotetrameric transmembrane glycoprotein consisting of two alpha and two beta subunits. It binds IGF-1 with high affinity, IGF2 with lower affinity, and Insulin with lowest affinity. IGF1R/Insulin R hybrids respond primarily to IGF-1 and may downregulate cellular responses to Insulin. IGF signaling is also modulated by IGF binding proteins and the scavenger receptor, IGF-II R/IGF2R. Mice lacking IGF1R show intrauterine growth deficiency and die at birth due to respiratory failure, and IGF1R mutations in humans can retarded pre- and postnatal growth. IGF-1 and its receptor are particularly important for neurogenesis, with deficiency producing microcephaly and learning disorders. IGF1R expression on human embryonic stem cells is important for their survival and clonogenicity.
Long Name
Insulin-like Growth Factor I Receptor
Alternate Names
CD221, IGF-1R, IGF-IR, IGF1R, IGFIR
Entrez Gene IDs
3480 (Human)
Gene Symbol
IGF1R
Additional IGF-I R/IGF1R Products
Product Documents for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
Product Specific Notices for Human Phospho-IGF-I R/IGF1R DuoSet IC ELISA
For research use only
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