Human PD-1 DuoSet ELISA

Name: Human PD-1 DuoSet ELISA
Brand: R&D Systems
SKU: DY1086

Catalog # DY1086 | R&D Systems, Inc. a Bio-Techne Brand

Citations (11)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	156 - 10,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human PD-1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (11) / Reviews

Summary for Human PD-1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PD-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human PD-1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human PD-1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Blocking Buffer:1% BSA in PBS, pH 7.2-7.4, 0.2 m filtered (R&D Systems Catalog # DY995). Quality of BSA is critical (see Technical Hints).

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

Scientific Data Examples for Human PD-1 DuoSet ELISA

	Human PD-1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-1

PD-1 (Programmed Death-1 receptor) is a key regulator of the threshold of immune response and peripheral immune tolerance. It is expressed on activated T cells, B cells, monocytes, and dendritic cells and binds to PD-L1 or PD-L2. PD-1 ligation induces co-inhibitory signals in T cells promoting their apoptosis, anergy, and functional exhaustion. Blockade of the PD-1: PD-L1 interaction using anti-PD-1 antibodies enhances understanding of anti-tumor immunity and advances the potential for cancer immunotherapy.

Item	Value
Long Name	Programmed Death-1
Entrez Gene IDs	5133 (Human); 18566 (Mouse); 301626 (Rat); 100533201 (Porcine); 486213 (Canine); 102123659 (Cynomolgus Monkey)
Alternate Names	CD279, CD279 antigen, PD-1, PD1, PD1hPD-l, PDCD1, Protein PD-1, SLEB2, hPD-1, programmed cell death 1, programmed cell death protein 1

Citations for Human PD-1 DuoSet ELISA (11)

Showing 1 - 10 of 11 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human PD-1 DuoSet ELISA include:

Species: Human
Sample Types: Plasma

K Krueger et al. (2022-10-11), Robust Preanalytical Performance of Soluble PD-1, PD-L1 and PD-L2 Assessed by Sensitive ELISAs in Blood Biomedicines, 2022-110(10).
PMID: 36289796
Species: Human
Sample Types: Plasma

K Krueger et al. (2022-09-26), High Quality Performance of Novel Immunoassays for the Sensitive Quantification of Soluble PD-1, PD-L1 and PD-L2 in Blood Biomedicines, 2022-010(10).
PMID: 36289665
Species: Human
Sample Types: Serum

N Tan et al. (2021-12-18), High Levels of Soluble Programmed Death-1 Are Associated with Virological Response in Chronic Hepatitis B patients after Antiviral Treatment Virus research, 2021-10(0):198660.
PMID: 34929214
Species: Human
Sample Types: Plasma

R Ohkuma et al. (2021-12-16), Increased Plasma Soluble PD-1 Concentration Correlates with Disease Progression in Patients with Cancer Treated with Anti-PD-1 Antibodies Biomedicines, 2021-19(12).
PMID: 34944745
Species: Human
Sample Types: Plasma

G Larrinaga et al. (2021-02-07), Soluble PD-L1 Is an Independent Prognostic Factor in Clear Cell Renal Cell Carcinoma Cancers, 2021-013(4).
PMID: 33562338
Species: Human
Sample Types: Serum

H Vajavaara et al. (2021-01-22), Soluble PD-1 but Not PD-L1 Levels Predict Poor Outcome in Patients with High-Risk Diffuse Large B-Cell Lymphoma Cancers, 2021-013(3).
PMID: 33499013
Species: Human
Sample Types: Cell Culture Lysates

ALD Pace et al. (2020-03-12), Characterization of Human NK Cell-Derived Exosomes: Role of DNAM1 Receptor In Exosome-Mediated Cytotoxicity Against Tumor Cancers (Basel), 2020-012(3).
PMID: 32178479
Species: Human
Sample Types: Serum

Y Guo et al. (2018-02-28), Immune checkpoint inhibitor PD-1 pathway is down-regulated in synovium at various stages of rheumatoid arthritis disease progression PLoS ONE, 2018-013(2):e0192704.
PMID: 29489833
Species: Human
Sample Types: Serum

S Kruger et al. (2017-03-31), Serum levels of soluble programmed death protein 1 (sPD-1) and soluble programmed death ligand 1 (sPD-L1) in advanced pancreatic cancer Oncoimmunology, 2017-06(5):e1310358.
PMID: 28638732
Species: Mouse
Sample Types: Cell Culture Supernates

MY Bartee et al. (2017-03-17), Tumor localized secretion of soluble PD1 enhances oncolytic virotherapy Cancer Res, 2017-00(0).
PMID: 28314785
Species: Human
Sample Types: Plasma

Cheng H et al. (2014-11-26), Circulating programmed death-1 as a marker for sustained high hepatitis B viral load and risk of hepatocellular carcinoma. PLoS ONE, 2014-19(11):e95870.
PMID: 25427199

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