Human LIF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # DLF00B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
5.36 pg/mL
Assay Range
31.3-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human LIF Quantikine ELISA Kit
The Quantikine® Human LIF Immunoassay is a 4.5 hours solid phase ELISA designed to measure human LIF in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human LIF and antibodies raised against the recombinant protein. Results obtained for naturally occurring human LIF showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human LIF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human LIF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 255 | 778 | 1433 | 268 | 827 | 1548 |
| Standard Deviation | 11.0 | 21.8 | 32.6 | 21.0 | 50.9 | 76.7 |
| CV% | 4.3 | 2.8 | 2.3 | 7.8 | 6.2 | 5.0 |
Recovery for Human LIF Quantikine ELISA Kit
The recovery of human LIF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 92-112 |
| EDTA Plasma (n=4) | 90 | 83-96 |
| Heparin Plasma (n=4) | 90 | 83-99 |
| Serum (n=4) | 92 | 83-101 |
Linearity
<div>To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LIF were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. </div>
Scientific Data Images for Human LIF Quantikine ELISA Kit
Human LIF ELISA Cell Culture Supernate Standard Curve
Human LIF ELISA Standard Curve
Human LIF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: LIF
LIF (Leukemia Inhibitory Factor) is a pleiotropic member of the IL-6 family of cytokines. It functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha/CD118, and a signal transducing subunit, gp130. gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27. LIF supports uterine implantation of the embryo, pluripotency in embryonic stem cells, and proliferation of progenitor cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, and it is required by the thymic epithelium to support T cell maturation. It promotes motor neuron survival and oligodendrocyte myelination, enhances adrenal production of cortisol and aldosterone, and promotes differentiation of adipocytes and cardiac smooth muscle cells. LIF can also function as an autocrine growth factor in pancreatic cancer and can induce formation of immunosuppressive tumor-associated macrophages.
Long Name
Leukemia Inhibitory Factor
Alternate Names
D Factor, Emfilermin, HILDA, MLPLI
Gene Symbol
LIF
Additional LIF Products
Product Documents for Human LIF Quantikine ELISA Kit
Product Specific Notices for Human LIF Quantikine ELISA Kit
For research use only
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