Human Intelectin-1/Omentin DuoSet ELISA
R&D Systems, part of Bio-Techne | Catalog # DY4254-05
Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
187-12000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Human
Human Intelectin-1/Omentin DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Product Summary for Human Intelectin-1/Omentin DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Intelectin-1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as tissue lysates, serum, and plasma, should be evaluated prior to use in this DuoSet ELISA.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Human Intelectin-1/Omentin DuoSet ELISA
Human Intelectin-1/Omentin DuoSet ELISA Standard Curve
Kit Contents for Human Intelectin-1/Omentin DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates):(Catalog #DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS:(Catalog #DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer:(Catalog #WA126), or 0.05% Tween®20 in PBS, pH 7.2-7.4
Reagent Diluent:0.1% BSA, 0.05% Tween 20 in Tris-buffered Saline (20 mM Trizma base, 150 mM NaCI) pH 7.2-7.4, 0.2 μm filtered
Substrate Solution:1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog #DY999)
Stop Solution:2 N H2SO4(Catalog #DY994)
Microplates: R&D Systems (Catalog #DY990)
Plate Sealers:ELISA Plate Sealers (Catalog #DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Intelectin-1/Omentin
Intelectin-1, also known as Omentin, HL-1, and Lactoferrin Receptor, is a GPI-linked protein that is expressed by vascular smooth muscle and endothelial cells, particularly in the heart, small intestine, colon, thymus, ovary, testis, and omental fat as well as by intestinal Paneth cells and airway and intestinal goblet cells. Intelectin-1 limits multiple aspects of cardiovascular pathology following ischemic injury. In addition, it inhibits osteoblast bone matrix mineralization, enhances insulin-stimulated uptake by adipocytes, supports neural stem cell viability, and inhibits the invasion and metastasis of neuroblastoma cells. Intelectin-1 binds to microbial galactofuranosyl carbohydrates and functions as a receptor for the iron-transporting protein Lactoferrin.
Alternate Names
HL-1, Intelectin1, ITLN1, Itlna, LFR, Omentin, Omentin-1
Gene Symbol
ITLN1
Additional Intelectin-1/Omentin Products
Product Documents for Human Intelectin-1/Omentin DuoSet ELISA
Product Specific Notices for Human Intelectin-1/Omentin DuoSet ELISA
For research use only
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