Human IL-7 Quantikine HS ELISA Kit Best Seller

Name: Human IL-7 Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HS750
Rating: 5 (2 reviews)

Catalog # HS750 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (44)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	18-24 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity:	0.1 pg/mL
Assay Range:	0.156 - 10 pg/mL (Cell Culture Supernates) 0.25 - 16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
Human IL-7 Quantikine HS ELISA Kit Summary:	The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.

View Full Technical Details for Human IL-7 Quantikine HS ELISA Kit

Technical Data
Citations (44) / Reviews (2)

Product Specifications for Human IL-7 Quantikine HS ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-7
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	0.5	1.49	6.86	0.78	2.13	8.81
Standard Deviation	0.04	0.14	0.61	0.08	0.16	0.64
CV%	8	9.4	8.9	10.3	7.5	7.3

Serum, EDTA Plasma, Citrate Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	0.46	5.79	11.18	0.51	6.38	12.95
Standard Deviation	0.03	0.19	0.44	0.06	0.5	1.3
CV%	6.5	3.3	3.9	11.8	7.8	10

Recovery

The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=5)	103	89-113
Citrate Plasma (n=5)	100	92-110
EDTA Plasma (n=5)	112	83-132
Serum (n=5)	100	91-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human IL-7 Quantikine HS ELISA Kit

	Human IL-7 High Sensitivity ELISA Cell Culture Supernate Standard Curve
	Human IL-7 High Sensitivity ELISA Serum/Plasma Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Item	Value
Long Name	Interleukin 7
Entrez Gene IDs	3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names	IL-7, IL-7interleukin-7, IL7, Lymphopoietin-1, PBGF, interleukin 7

Citations for Human IL-7 Quantikine HS ELISA Kit (44)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-7 Quantikine HS ELISA Kit include:

Species: Human
Sample Types: Cell Culture Supernates

Y Kitamura et al. (2023-01-06), Roles of IL-7R Induced by Interactions between Cancer Cells and Macrophages in the Progression of Esophageal Squamous Cell Carcinoma Cancers, 2023-015(2).
PMID: 36672342
Species: Human
Sample Types: Serum

C Cuéllar et al. (2022-08-11), Association between anti-Anisakis simplex antibodies and interleukin-7 levels International immunopharmacology, 2022-0111(0):109134.
PMID: 35964412
Species: Human
Sample Types: Plasma

T Sainz et al. (2022-02-26), Targeting the Gut Microbiota of Vertically HIV-Infected Children to Decrease Inflammation and Immunoactivation: A Pilot Clinical Trial Nutrients, 2022-014(5).
PMID: 35267967
Species: Human
Sample Types: Plasma

C Bacchus-So et al. (2021-01-19), Relationship between CD4 T cell turnover, cellular differentiation and HIV persistence during ART PloS Pathogens, 2021-017(1):e1009214.
PMID: 33465157
Species: Human
Sample Types: Plasma

J Xun et al. (2020-10-19), Mycobacterium tuberculosis co-infection is associated with increased surrogate marker of the HIV reservoir AIDS Res Ther, 2020-117(1):63.
PMID: 33076959
Species: Human
Sample Types: Cell Culture Supernates

Y Yuan et al. (2019-09-12), HIV-1 Tat protein inhibits the hematopoietic support function of human bone marrow mesenchymal stem cells Virus Res., 2019-0273(0):197756.
PMID: 31521762
Species: Human
Sample Types: Plasma

A Rajamanick et al. (2018-05-24), Altered levels of memory T cell subsets and common ?c cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment PLoS Negl Trop Dis, 2018-012(5):e0006481.
PMID: 29795573
Species: Human
Sample Types: Serum

J Seyfarth et al. (2018-03-13), IL7RA haplotypes differentially affect soluble IL-7 receptor and IL-7 serum concentrations in children with type 1 diabetes Pediatr Diabetes, 2018-00(0).
PMID: 29484785
Species: Human
Sample Types: Plasma

C Lundtoft et al. (2017-06-05), Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis PLoS Pathog., 2017-013(6):e1006425.
PMID: 28582466
Species: Primate
Sample Types: Tissue Homogenates

R Ponte et al. (2017-05-19), Acute Simian Immunodeficiency Virus Infection Triggers Early and Transient Interleukin-7 Production in the Gut, Leading to Enhanced Local Chemokine Expression and Intestinal Immune Cell Homing Front Immunol, 2017-08(0):588.
PMID: 28579989
Species: Human
Sample Types: Plasma

NP Kumar et al. (2017-04-27), Diminished plasma levels of common ?-chain cytokines in pulmonary tuberculosis and reversal following treatment PLoS ONE, 2017-012(4):e0176495.
PMID: 28448542
Species: Human
Sample Types: Cell Culture Supernates

P Severino et al. (2017-02-14), Human Lymph Node-Derived Fibroblastic and Double-Negative Reticular Cells Alter Their Chemokines and Cytokines Expression Profile Following Inflammatory Stimuli Front Immunol, 2017-08(0):141.
PMID: 28261205
Species: Human
Sample Types: Cell Culture Supernates

(2016-09-02), Establishment of IL-7 Expression Reporter Human Cell Lines, and Their Feasibility for High-Throughput Screening of IL-7-Upregulating Chemicals PLoS ONE, 2016-011(9):e0161899.
PMID: 27589392
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma

Okoye A et al. (2015-09-28), Effect of IL-7 Therapy on Naive and Memory T Cell Homeostasis in Aged Rhesus Macaques. J Immunol, 2015-0195(9):4292-305.
PMID: 26416281
Species: Human
Sample Types: Cell Culture Supernates

Bazdar D et al. (2015-04-13), Recycled IL-7 Can Be Delivered to Neighboring T Cells. J Immunol, 2015-0194(10):4698-704.
PMID: 25870237
Species: Human
Sample Types: Plasma

Hannon M et al. (2015-03-16), Immune Recovery after Allogeneic Hematopoietic Stem Cell Transplantation Following Flu-TBI versus TLI-ATG Conditioning. Clin Cancer Res, 2015-021(14):3131-9.
PMID: 25779951
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Plasma

Bruel T et al. (2015-01-14), Long-term control of simian immunodeficiency virus (SIV) in cynomolgus macaques not associated with efficient SIV-specific CD8+ T-cell responses. J Virol, 2015-089(7):3542-56.
PMID: 25589645
Species: Human
Sample Types: Plasma

Kielsen K et al. (2015-01-01), T cell reconstitution in allogeneic haematopoietic stem cell transplantation: prognostic significance of plasma interleukin-7. Scand J Immunol, 2015-081(1):72-80.
PMID: 25263171
Species: Human
Sample Types: Cell Culture Supernates

Cha L et al. (2014-07-25), IFN-alpha exerts opposing effects on activation-induced and IL-7-induced proliferation of T cells that may impair homeostatic maintenance of CD4+ T cell numbers in treated HIV infection. J Immunol, 2014-0193(5):2178-86.
PMID: 25063872
Species: Human
Sample Types: Serum

Le Saout C et al. (2014-03-06), Chronic exposure to type-I IFN under lymphopenic conditions alters CD4 T cell homeostasis. PLoS Pathog, 2014-010(3):e1003976.
PMID: 24603698
Species: Human
Sample Types: Cell Culture Supernates

Loubaki L et al. (2013-12-05), Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature. PLoS ONE, 2013-18(12):e81983.
PMID: 24349168
Species: Human
Sample Types: Plasma

Zheng M et al. (2013-03-27), The systemic cytokine environment is permanently altered in multiple myeloma. PLoS ONE, 2013-08(3):e58504.
PMID: 23544044
Species: Human
Sample Types: Cell Culture Supernates

Cai et al. (2013-03-12), Up-regulation of intestinal epithelial cell derived IL-7 expression by keratinocyte growth factor through STAT1/IRF-1, IRF-2 pathway. PLoS ONE, 2013-08(3):e58647.
PMID: 23554911
Species: Human
Sample Types: Plasma

(2012-08-23), Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients. Genes Immun, 2012-013(7):587-92.
PMID: 22914435
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma

Vassena L et al. (2012-04-12), Treatment with IL-7 prevents the decline of circulating CD4+ T cells during the acute phase of SIV infection in rhesus macaques. PLoS Pathog., 2012-08(4):e1002636.
PMID: 22511868
Species: Human
Sample Types: Serum

Steel C et al. (2011-04-29), Altered T cell memory and effector cell development in chronic lymphatic filarial infection that is independent of persistent parasite antigen. PLoS ONE, 2011-06(4):e19197.
PMID: 21559422
Species: Human
Sample Types: Plasma

Thiant S et al. (2010-12-06), Plasma levels of IL-7 and IL-15 after reduced intensity conditioned allo-SCT and relationship to acute GVHD. Bone Marrow Transplant., 2010-146(10):1374-81.
PMID: 21132028
Species: Human
Sample Types: Plasma

Vyth-Dreese FA et al. (2010-10-05), Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin. Exp. Immunol., 2010-1162(3):447-59.
PMID: 20942806
Species: Human
Sample Types: Serum

Antonelli LR et al. (2010-07-26), Elevated frequencies of highly activated CD4+ T cells in HIV+ patients developing immune reconstitution inflammatory syndrome. Blood, 2010-0116(19):3818-27.
PMID: 20660788
Species: Human
Sample Types: Serum

Sugalski JM et al. (2010-07-23), Peripheral blood B cell subset skewing is associated with altered cell cycling and intrinsic resistance to apoptosis and reflects a state of immune activation in chronic hepatitis C virus infection. J. Immunol., 2010-0185(5):3019-27.
PMID: 20656924
Species: Human
Sample Types: Plasma

Thiant S et al. (2010-03-01), Plasma levels of IL-7 and IL-15 in the first month after myeloablative BMT are predictive biomarkers of both acute GVHD and relapse. Bone Marrow Transplant., 2010-045(10):1546-52.
PMID: 20190846
Species: Human
Sample Types: Plasma

Juffroy O et al. (2010-01-01), Dual mechanism of impairment of interleukin-7 (IL-7) responses in human immunodeficiency virus infection: decreased IL-7 binding and abnormal activation of the JAK/STAT5 pathway. J. Virol., 2010-084(1):96-108.
PMID: 19864382
Species: Human
Sample Types: Cell Culture Supernates

Cattaruzza L et al. (2009-09-01), Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma. Int. J. Cancer, 2009-0125(5):1092-101.
PMID: 19391137
Species: Human
Sample Types: Plasma

Rose T et al. (2009-06-15), Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients. J. Immunol., 2009-0182(12):7389-97.
PMID: 19494261
Species: Human
Sample Types: Synovial Fluid

Rihl M et al. (2008-11-01), Identification of interleukin-7 as a candidate disease mediator in spondylarthritis. Arthritis Rheum., 2008-158(11):3430-5.
PMID: 18975340
Species: Human
Sample Types: Plasma

Louis CU et al. (2008-10-29), Enhancing the in vivo expansion of adoptively transferred EBV-specific CTL with lymphodepleting CD45 monoclonal antibodies in NPC patients. Blood, 2008-1113(11):2442-50.
PMID: 18971421
Species: Human
Sample Types: Plasma

Mancebo E et al. (2008-09-22), Longitudinal analysis of immune function in the first 3 years of life in thymectomized neonates during cardiac surgery. Clin. Exp. Immunol., 2008-0154(3):375-83.
PMID: 18811694
Species: Human
Sample Types: Serum

Benedict C et al. (2007-05-23), Sleep enhances serum interleukin-7 concentrations in humans. Brain Behav. Immun., 2007-021(8):1058-62.
PMID: 17524612
Species: Human
Sample Types: Serum

Colucci S et al. (2007-05-01), Lymphocytes and synovial fluid fibroblasts support osteoclastogenesis through RANKL, TNFalpha, and IL-7 in an in vitro model derived from human psoriatic arthritis. J. Pathol., 2007-0212(1):47-55.
PMID: 17370327
Species: Human
Sample Types: Serum

Fluur C et al. (2007-04-15), Potential role for IL-7 in Fas-mediated T cell apoptosis during HIV infection. J. Immunol., 2007-0178(8):5340-50.
PMID: 17404319
Species: Human
Sample Types: Plasma

Koesters SA et al. (2006-02-01), IL-7Ralpha expression on CD4+ T lymphocytes decreases with HIV disease progression and inversely correlates with immune activation. Eur. J. Immunol., 2006-036(2):336-44.
PMID: 16421946
Species: Human
Sample Types: Cell Culture Supernates

Johnson SE et al. (2005-12-01), Murine and human IL-7 activate STAT5 and induce proliferation of normal human pro-B cells. J. Immunol., 2005-1175(11):7325-31.
PMID: 16301638
Species: Primate - Cebus capucinus (White-headed Capuchin Monkey), Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Plasma

Muthukumar A et al. (2003-10-02), Elevated interleukin-7 levels not sufficient to maintain T-cell homeostasis during simian immunodeficiency virus-induced disease progression. Blood, 2003-1103(3):973-9.
PMID: 14525780
Species: Primate - Papio cynocephalus (Yellow Baboon)
Sample Types: Serum

Storek J et al. (2003-01-23), Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys. Blood, 2003-0101(10):4209-18.
PMID: 12543864

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Human IL-7 Quantikine HS ELISA Kit

Name: Alisha Freeman

Sample Tested: CHO Chinese hamster ovary cell line

Verified Customer | Posted 07/11/2018
Human IL-7 Quantikine HS ELISA Kit

Name: Prakasha Kempaiah

Sample Tested: Serum

Verified Customer | Posted 06/03/2016

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