Human IL-18/IL-18 BPa Complex DuoSet ELISA

Name: Human IL-18/IL-18 BPa Complex DuoSet ELISA
Brand: R&D Systems
SKU: DY8936-05
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # DY8936-05

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Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	62.5 - 4,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human IL-18/IL-18 BPa Complex DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Summary for Human IL-18/IL-18 BPa Complex DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Interleukin 18/Interleukin 18 Binding Protein isoform a Complex (IL-18/IL-18 BPa Complex). The suggested diluent is suitable for the analysis of most cell culture supernate, serum, and plasma samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human IL-18/IL-18 BPa Complex DuoSet ELISA

	Human IL-18 / IL-18 BPa Complex ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18/IL-18 BPa Complex

Interleukin 18 (IL-18), also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, is a cytokine which shares biologic activities with IL-12 and structural similarities with the IL-1 family of proteins. IL-18 was originally cloned from liver cells and has since been shown to be expressed by monocyte/macrophages, osteoblasts and keratinocytes. Caspase-1 (IL-1 beta-converting enzyme) has been implicated in the physiological processing of pro-IL-18 to IL-18. Similarly to IL-12, human IL-18 has been shown to enhance NK cell activity in PBMC cultures. Human IL-18 has also been found to induce the production IFN-γ and GM-CSF while inhibiting the production of IL-10 by PBMC. On enriched human T cells, human IL-18 can enhance Th1 cytokine production and stimulate cell proliferation via an IL-2-dependent pathway. In the mouse system, IL-18 has been shown to be a costimulatory factor for the activation of Th1, but not Th2, cells. IL-18 was found to selectively enhance the FasL-mediated cytotoxicity of Th1, but not Th0 or Th2, cells. IL-18 has also been shown to induce activated B cells to produce IFN-γ that inhibits IgE production.

Item	Value
Long Name	Interleukin 18/Interleukin 18 Binding Protein Isoform a Complex
Entrez Gene IDs	10068 (Human)
Alternate Names	IL-18/IL-18 BPa Complex

Citations for Human IL-18/IL-18 BPa Complex DuoSet ELISA (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-18/IL-18 BPa Complex DuoSet ELISA include:

Species: Human
Sample Types: Serum

B Kronsteine et al. (2019), Diabetes alters immune response patterns to acute melioidosis in humans Eur. J. Immunol., 0(0).
PMID: 31032897

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Human IL-18/IL-18 BPa Complex DuoSet ELISA

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 08/11/2017

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Product Documents for Human IL-18/IL-18 BPa Complex DuoSet ELISA

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Human IL-18/IL-18 BPa Complex DuoSet ELISA

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Summary for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Product Specifications for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Kit Contents for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Preparation and Storage

Background: IL-18/IL-18 BPa Complex

Citations for Human IL-18/IL-18 BPa Complex DuoSet ELISA (1)

Customer Reviews for Human IL-18/IL-18 BPa Complex DuoSet ELISA (1)

Customer Images

Human IL-18/IL-18 BPa Complex DuoSet ELISA

Product Documents for Human IL-18/IL-18 BPa Complex DuoSet ELISA

Product Datasheets

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