Human HGF Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DHG00B
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL)
Sensitivity
24.1 pg/mL
Assay Range
156-10000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Product Summary for Human HGF Quantikine ELISA Kit
The Quantikine® Human HGF Immunoassay is a 4.0 hour solid phase ELISA that is designed to measure human HGF levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human HGF and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human HGF. Results obtained using natural human HGF showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human HGF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human HGF.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 1296 | 2626 | 5080 | 1414 | 2755 | 5410 |
| Standard Deviation | 30.2 | 75.8 | 147 | 112 | 176 | 212 |
| CV% | 2.3 | 2.9 | 2.9 | 7.9 | 6.4 | 3.9 |
Recovery for Human HGF Quantikine ELISA Kit
The recovery of human HGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 107 | 101-118 |
| Citrate Plasma (n=4) | 102 | 91-115 |
| EDTA Plasma (n=4) | 104 | 95-114 |
| Heparin Plasma (n=4) | 113 | 96-125 |
| Serum (n=4) | 104 | 94-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human HGF were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human HGF Quantikine ELISA Kit
Human HGF ELISA Cell Culture Supernate Standard Curve
Human HGF ELISA Standard Curve
Human HGF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: HGF
HGF (Hepatocyte Growth Factor, Scatter Factor) induces the proliferation and migration of epithelial cells, hepatocytes, chondrocytes, keratinocytes, melanocytes, endothelial cells, and many tumor cells. During organogenesis and tissue repair, HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells, neuronal survival, and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage, acute kidney failure, myocardial infarction, type 1 diabetes, obesity, and cancer, as well as in the synovial fluid of rheumatoid arthritis patients.
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
Additional HGF Products
Product Documents for Human HGF Quantikine ELISA Kit
Product Specific Notices for Human HGF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.