Human Flt-3/Flk-2 DuoSet ELISA

Name: Human Flt-3/Flk-2 DuoSet ELISA
Brand: R&D Systems
SKU: DY812-05

Catalog # DY812-05 | R&D Systems, Inc. a Bio-Techne Brand

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	125 - 8,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human Flt-3/Flk-2 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

View Full Technical Details for Human Flt-3/Flk-2 DuoSet ELISA

Technical Data
Citations / Reviews

Summary for Human Flt-3/Flk-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Flt-3 / Flk-2 Ectodomain. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications for Human Flt-3/Flk-2 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For five 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human Flt-3/Flk-2 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data Examples for Human Flt-3/Flk-2 DuoSet ELISA

	Human Flt-3 / Flk-2 Ectodomain ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Flt-3/Flk-2

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and STK-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes c-kit, the receptor for SCF, and fms, the receptor for M-CSF. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin.

Item	Value
Long Name	fms-like Tyrosine Kinase 3
Entrez Gene IDs	2322 (Human); 14255 (Mouse)
Alternate Names	CD135, CD135 antigen, EC 2.7.10, FL cytokine receptor, FLK2, FLT3 receptor tyrosine kinase, Flk-2, Flt-3, Flt3, Fms-like tyrosine kinase 3, STK-1, STK1EC 2.7.10.1, Stem cell tyrosine kinase 1, Tyrosine-protein kinase receptor FLT3, fetal liver kinase 2, fms-related tyrosine kinase 3, growth factor receptor tyrosine kinase type III

Citations for Human Flt-3/Flk-2 DuoSet ELISA

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Human Flt-3/Flk-2 DuoSet ELISA

Catalog # DY812-05 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary for Human Flt-3/Flk-2 DuoSet ELISA

Product Specifications for Human Flt-3/Flk-2 DuoSet ELISA

Kit Contents for Human Flt-3/Flk-2 DuoSet ELISA

Other Reagents Required

Scientific Data Examples for Human Flt-3/Flk-2 DuoSet ELISA

Preparation and Storage

Background: Flt-3/Flk-2

Citations for Human Flt-3/Flk-2 DuoSet ELISA

Product Documents for Human Flt-3/Flk-2 DuoSet ELISA

Product Datasheets

COA

Certificate of Analysis

SDS

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