Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
R&D Systems, part of Bio-Techne | Catalog # DEPRU0B
Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
Key Product Details
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
0.221 mIU/mL
Assay Range
2.5-200 mIU/mL (Serum, EDTA Plasma)
Product Summary for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
The Quantikine Human Epo Immunoassay is a 2.5 hour solid phase immunoassay designed to measure human Epo in serum and EDTA plasma. It contains recombinant human Epo, and antibodies raised against the recombinant protein. Natural human Epo showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human Epo.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Epo.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess Intra-Assay Precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess Inter-Assay Precision. Assays were performed by at least three technicians using two lots of components.
EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (mIU/mL) | 10.3 | 40.3 | 117 | 10.4 | 41.9 | 118 |
| Standard Deviation | 0.370 | 1.45 | 1.97 | 0.860 | 2.20 | 5.78 |
| CV% | 3.6 | 3.6 | 1.7 | 8.3 | 5.3 | 4.9 |
Recovery for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
The recovery of human Epo spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 94 | 84-98 |
| Serum (n=4) | 101 | 96-107 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Epo in various matrices were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
Human Erythropoietin Quantikine ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Erythropoietin/EPO
Erythropoietin/EPO is a renal glycoprotein hormone that promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha. Erythropoietin/EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis.
R&D System products include Erythropoietin antibodies, proteins, and ELISAs. Our products cover multiple species and our EPO antibodies are validated for multiple applications including Neutralization, Western blot, ELISA, and Immunohistochemistry.
Alternate Names
ECYT5, EPO, MVCD2
Gene Symbol
EPO
Additional Erythropoietin/EPO Products
Product Documents for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
Product Specific Notices for Human Erythropoietin/EPO Quantikine ELISA Kit, RUO
For research use only
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