Human ErbB2/Her2 Quantikine ELISA Kit

Name: Human ErbB2/Her2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: DHER20

Catalog # DHER20 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

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Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (50 uL)
Sensitivity:	14.8 pg/mL
Assay Range:	78.1 - 5,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk)
Human ErbB2/Her2 Quantikine ELISA Kit Summary:	The Quantikine Human ErbB2/Her2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB2 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human ErbB2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ErbB2.

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Technical Data
Citations (1) / Reviews

Product Specifications for Human ErbB2/Her2 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human ErbB2.
Cross-reactivity	Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	471	1379	2681	489	1419	2667
Standard Deviation	6.71	16.3	31.1	18.7	56.4	93.5
CV%	1.4	1.2	1.2	3.8	4	3.5

Recovery

The recovery of human ErbB2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	90-120
EDTA Plasma (n=4)	99	89-118
Heparin Plasma (n=4)	104	92-119
Serum (n=4)	99	91-115
Urine (n=4)	101	94-106

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human ErbB2/Her2 Quantikine ELISA Kit

	Human ErbB2/Her2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Item	Value
Long Name	Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs	2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names	CD340, CD340 antigen, EC 2.7.10, EGFR2, ErbB2, HER-2, HER2, HER2EC 2.7.10.1, MLN 19, MLN19, Metastatic lymph node gene 19 protein, NEUHER-2/neu, NGL, NGLTKR1, Neu Oncogene, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, herstatin, neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, receptor tyrosine-protein kinase erbB-2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Human ErbB2/Her2 Quantikine ELISA Kit (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human ErbB2/Her2 Quantikine ELISA Kit include:

Species: Human
Sample Types: Serum

V Agnolon et al. (2020-02-20), ELISA assay employing epitope-specific monoclonal antibodies to quantify circulating HER2 with potential application in monitoring cancerï¿½patients undergoing therapy with trastuzumab Sci Rep, 2020-010(1):3016.
PMID: 32080226

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Product Documents for Human ErbB2/Her2 Quantikine ELISA Kit

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COA

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.