Human EpCAM/TROP1 DuoSet ELISA

Name: Human EpCAM/TROP1 DuoSet ELISA
Brand: R&D Systems
SKU: DY960

Catalog # DY960 | R&D Systems, Inc. a Bio-Techne Brand

Citations (2)

Product Datasheet / COA / SDS

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Key Product Details

Assay Type:	Solid Phase Sandwich ELISA
Assay Range:	93.8 - 6,000 pg/mL
Sample Types:	Cell culture supernates, serum, and plasma Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Human EpCAM/TROP1 DuoSet ELISA Features:	Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time Development protocols are provided to guide further assay optimization Assay can be customized to your specific needs Economical alternative to complete kits

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Technical Data
Citations (2) / Reviews

Summary for Human EpCAM/TROP1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human EpCAM/TROP1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Specifications for Human EpCAM/TROP1 DuoSet ELISA

Assay Format	96-well strip plate (sold separately)
Sample Volume Required	100 µL
Reactivity	Human
Specificity	Please see the product datasheet
Detection Method	Colorimetric ELISA - 450nm (TMB)
Conjugate	Biotin
Label	HRP
Measurement	Quantitative ELISA
Sufficient Materials	For fifteen 96-well plates*

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Kit Contents for Human EpCAM/TROP1 DuoSet ELISA

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Scientific Data Examples for Human EpCAM/TROP1 DuoSet ELISA

	Human EpCAM / TROP1 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as TROP 1, is a calcium-dependent homophilic adhesion protein. It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1. During embryonic development, EpCAM is expressed in lung, kidney, liver, pancreas, skin, and germ cells. In the adult, it is expressed on basolateral cell membranes of simple, pseudo-stratified, and transitional epithelia. EpCAM is upregulated in proliferating epithelial tissues, during adult liver regeneration, and many carcinomas.

Item	Value
Long Name	Epithelial Cell Adhesion Molecule
Entrez Gene IDs	4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)
Alternate Names	17-1A, 323/A3, ACSTD1, CD326, CD326 antigen, Cell surface glycoprotein Trop-1, DIAR5, EGP, EGP-2, EGP314, EGP40, ESA, EpCAM, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, GA733-2, GA733-2EGP34, HNPCC8, KS 1/4 antigen, KS1/4, KSAHEA125, M1S2, M4S1, M4S1Ly74, MIC18MH99, MOC31, Major gastrointestinal tumor-associated protein GA733-2, TACST-1, TACSTD1, TROP1, TROP1CD326, Tumor-associated calcium signal transducer 1CO-17A, antigen identified by monoclonal AUA1, chromosome 4, surface marker (35kD glycoprotein), epithelial cell adhesion molecule, gp40, hEGP314

Citations for Human EpCAM/TROP1 DuoSet ELISA (2)

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Citations are publications that use Bio-Techne products. Selected citations for Human EpCAM/TROP1 DuoSet ELISA include:

Species: Human
Sample Types: Cell Culture Supernates

NV Sankpal et al. (2021-05-12), Cancer-associated mutations reveal a novel role for EpCAM as an inhibitor of cathepsin-L and tumor cell invasion BMC Cancer, 2021-021(1):541.
PMID: 33980181
Species: Human
Sample Types: Urine

Bryan et al. (2013-11-28), Urinary EpCAM in urothelial bladder cancer patients: characterisation and evaluation of biomarker potential. Br J Cancer, 2013-1110(3):679-85.
PMID: 24292452

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Product Documents for Human EpCAM/TROP1 DuoSet ELISA

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