Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
R&D Systems, part of Bio-Techne | Catalog # QK1146
Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
Key Product Details
Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Cell Lysates (100 µL), Cynomolgus Monkey EDTA Plasma (100 µL), Cynomolgus Monkey Serum (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)
Sensitivity
2.29 pg/mL
Assay Range
14.1-900 pg/mL (Cell Culture Supernates, Cell Lysates, Cynomolgus Monkey EDTA Plasma, Cynomolgus Monkey Serum, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
The Quantikine QuicKit Human/Cynomolgus Monkey PD-L1/B7-H1 Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human/cynomolgus monkey PD-L1/B7-H1 levels in cell culture supernates, serum, plasma, cell lysates, and urine. It contains recombinant human PD-L1/B7-H1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human/cynomolgus monkey PD-L1/B7-H1 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human/cynomolgus monkey PD-L1/B7-H1.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human, Cynomolgus Monkey
Specificity
Natural and recombinant human PD-L1/B7-H1 and natural cynomolgus monkey PD-L1/B7-H1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.
Cell Culture Supernates, Cell Lysates, Cynomolgus Monkey EDTA Plasma, Cynomolgus Monkey Serum, EDTA Plasma, Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (pg/mL) | 86.8 | 516 | 89.1 | 557 |
| Standard Deviation | 2.39 | 9.10 | 11.6 | 73.9 |
| CV% | 2.8 | 1.8 | 13.0 | 13.3 |
Recovery for Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
The recovery of PD-L1/B7-H1 is spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 108 | 105-111 |
| Cynomolgus Monkey EDTA Plasma (n=2) | 113 | 108-119 |
| Cynomolgus Monkey Serum (n=2) | 103 | 97-109 |
| EDTA Plasma (n=2) | 104 | 99-114 |
| Heparin Plasma (n=2) | 98 | 91-108 |
| Lysis Buffer (n=1) | 121 | 117-127 |
| Serum (n=2) | 103 | 96-110 |
| Urine (n=2) | 110 | 106-116 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PD-L1/B7-H1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PD-L1/B7-H1
PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 protein binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells. Many tumors overexpress PD-L1 protein making the PD-1:PD-L1 interaction central to several successful cancer immunotherapy approaches.
Long Name
Programmed Death Ligand 1
Alternate Names
B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Entrez Gene IDs
Gene Symbol
CD274
Additional PD-L1/B7-H1 Products
Product Documents for Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
Product Specific Notices for Human/Cynomolgus Monkey PD-L1/B7-H1 QuicKit ELISA
For research use only
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