Human CD25/IL-2 R alpha QuicKit ELISA

Name: Human CD25/IL-2 R alpha QuicKit ELISA
Brand: R&D Systems
SKU: QK0223

R&D Systems, part of Bio-Techne | Catalog # QK0223

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Product Datasheet / COA / SDS

Key Product Details

Assay Length:

80 minutes

Human CD25/IL-2 R alpha QuicKit ELISA Summary:

The Quantikine QuicKit Human CD25/IL-2 R alpha Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human IL-2 R alpha levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human IL-2 R alpha and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-2 R alpha showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-2 R alpha.

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View Full Technical Details for Human CD25/IL-2 R alpha QuicKit ELISA

Technical Data
Citations / Reviews

Product Specifications for Human CD25/IL-2 R alpha QuicKit ELISA

Measurement	Quantitative ELISA
Conjugate	HRP
Reactivity	Human
Specificity	Natural and recombinant human IL-2 R alpha.
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least four technicians

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	149	892	154	904
Standard Deviation	3.37	24.1	11.5	46.7
CV%	2.3	2.7	7.5	5.2

Recovery

The recovery of human IL-2 R alpha spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	100	93-105
EDTA Plasma (n=2)	93	83-97
Heparin Plasma (n=2)	92	84-97
Lysis Buffer (n=1)	101	96-106
Serum (n=2)	91	87-100

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-2 R alpha were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Human CD25/IL-2 R alpha QuicKit ELISA

	Human CD25/IL-2 R alpha Quantikine QuicKit ELISA Standard Curve

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD25/IL-2R alpha

CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.

Item	Value
Long Name	Interleukin 2 Receptor alpha
Entrez Gene IDs	3559 (Human); 16184 (Mouse); 25704 (Rat); 403870 (Canine); 102123605 (Cynomolgus Monkey)
Alternate Names	CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL-2Ra, IL2-RA, IL2R, IL2R alpha, IL2RA, TAC antigen, TCGFR, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55

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