Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit Best Seller
R&D Systems, part of Bio-Techne | Catalog # DBLYS0B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
6.44 pg/mL
Assay Range
62.5-4000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
The Quantikine Human BAFF/BLyS Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BAFF/BLyS in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human BAFF/BLyS and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human BAFF/BLyS showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BAFF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human BAFF/BLyS
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 433 | 1000 | 2343 | 474 | 1118 | 2111 |
| Standard Deviation | 28.2 | 72.0 | 78.6 | 46.8 | 112 | 244 |
| CV% | 6.5 | 7.2 | 3.4 | 9.9 | 10.0 | 11.6 |
Recovery for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
The recovery of BAFF/BLyS spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 96 | 90-103 |
| EDTA Plasma (n=4) | 94 | 84-101 |
| Heparin Plasma (n=4) | 94 | 89-107 |
| Serum (n=4) | 93 | 84-106 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of BAFF/BLyS were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Human BAFF/BLyS/TNFSF13B ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BAFF/BLyS/TNFSF13B
BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.
Long Name
B cell Activating Factor
Alternate Names
BLyS, CD257, TALL1, THANK, TNFSF13B, ZTNF4
Gene Symbol
TNFSF13B
Additional BAFF/BLyS/TNFSF13B Products
Product Documents for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
Product Specific Notices for Human BAFF/BLyS/TNFSF13B Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.