Human Albumin Quantikine QuicKit ELISA New
R&D Systems, part of Bio-Techne | Catalog # QK1455
Key Product Details
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL), Human Milk (10 µL), Cerebrospinal Fluid (10 µL)
Sensitivity
3.97 ng/mL
Assay Range
4.69-150 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk, Cerebrospinal Fluid)
Product Summary for Human Albumin Quantikine QuicKit ELISA
The Quantikine QuicKit Human Albumin Immunoassay is a one-step, 80-minute solid phase ELISA designed to measure human Albumin levels in cell culture supernates, serum, plasma, human milk, cerebrospinal fluid, saliva, and urine. It contains recombinant human Albumin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Albumin showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural human Albumin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Human
Specificity
Natural and recombinant human Albumin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision.
Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 20 | 20 | 10 | 10 |
| Mean (ng/mL) | 31.0 | 82.4 | 29.4 | 83.2 |
| Standard Deviation | 0.801 | 1.50 | 3.22 | 6.66 |
| CV% | 2.6 | 1.8 | 10.9 | 8.0 |
Recovery for Human Albumin Quantikine QuicKit ELISA
The recovery of Albumin is spiked to three different levels in samples throughout the range of the assay in cell culture media was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=2) | 122 | 117-127 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Albumin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human Albumin Quantikine QuicKit ELISA
Quantikine QuicKit Human Albumin ELISA Standard Curve
Human Albumin QuicKit Natural Linearity
Human Albumin QuicKit natural linearity testing against competitor kit.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Albumin
Albumin is the most abundant plasma protein, constituting approximately 60% of the total protein in blood. It is produced primarily by the liver and is important for the maintenance of colloidal osmotic blood pressure. Therefore, it maintains the balance of fluid between the vascular compartment and extravascular tissue. It also acts as a transport protein, carrying fatty acids, bilirubin, and various drugs and hormones.
Additional Albumin Products
Product Documents for Human Albumin Quantikine QuicKit ELISA
Product Specific Notices for Human Albumin Quantikine QuicKit ELISA
For research use only
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