Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49785

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all GRP75/HSPA9B ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human GRP75/HSPA9B/Mortalin in samples. No significant cross-reactivity or interference between Human GRP75/HSPA9B/Mortalin and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% < 10

Inter-Assay Precision (Precision between assays) CV% < 10

Recovery for Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)
Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

Scientific Data Images for Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

ELISA: Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric) [NBP3-49785]

Standard Curve Reference.

Kit Contents for Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: GRP75/HSPA9B

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human GRP75/HSPA9B/Mortalin ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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