Human Ephrin-B1 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42481
Key Product Details
Sample Type & Volume Required Per Well
Tissue homogenates, Cell lysates and other biological fluids (100 uL)
Sensitivity
0.055 ng/mL (example only; lot dependent)
Assay Range
0.156 - 10 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
Assay time: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Kit Contents for Human Ephrin-B1 ELISA Kit (Colorimetric)
- Assay Diluent A
- Assay Diluent B
- Detection Reagent A
- Detection Reagent B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated ready to use 96-well strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Ephrin-B1
Ephrins are naturally divided into two structural groups. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor-binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins, which can be tyrosine phosphorylated upon receptor ligation. Class B ephrins show 33% amino acid sequence identity in their extracellular segments and 44% amino acid sequence identity in their cytoplasmic regions.
Alternate Names
Cek5-L, EFL-3, EFNB1, ELK-L, EphrinB1, LERK-2, STRA-1
Gene Symbol
EFNB1
Additional Ephrin-B1 Products
Product Documents for Human Ephrin-B1 ELISA Kit (Colorimetric)
Product Specific Notices for Human Ephrin-B1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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