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DuoSet ELISA Ancillary Reagent Kit 2

R&D Systems, Inc. a Bio-Techne Brand

Product in process of being discontinued and replaced by new Next generation kit

Key Product Details

R&D Systems DuoSet Ancillary Reagent Kits
Discontinued Product
DY008 has been discontinued and is replaced by DY008B.
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Citations for DuoSet ELISA Ancillary Reagent Kit 2 (68)

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Customer Reviews for DuoSet ELISA Ancillary Reagent Kit 2 (90)

4.9 out of 5
90 customer ratings
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  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Gabriele Strusi
    Verified Customer | Posted 11/21/2022
    HepG2 conditioned medium
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 11/02/2022
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 11/02/2022
    LOW BLANK SIGNAL. HENCE EXCELLENT.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 06/18/2022
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/31/2022
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/26/2022
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 03/24/2022
    Works very well if protocol is followed. Another high binding plates can also be used optionally
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: James Williams
    Verified Customer | Posted 03/16/2022
    Budget friendly and produces excellent results
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Troy Trevino
    Verified Customer | Posted 03/11/2022
    Used with Mouse CXCL10 ELISA Kit DY446
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 12/16/2021
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 12/11/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Rob Knight
    Verified Customer | Posted 12/09/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/06/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/28/2021
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/19/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/06/2021
    Very good kit. It’s reasonable to buy this kit instead of buying everything separately. The result is reproducible.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/06/2021
    This kit is very helpful. The protocol is also easy and straight forward
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/18/2021
    super convenient and budge friendly
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 04/09/2021
    Results are very reproducible and the kit was very helpful and easy to use. This saved a lot of time, with minimal troubleshooting.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 04/09/2021
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Arun Kashyap
    Verified Customer | Posted 12/20/2020
    The results are reproducible between different Lots of this kit.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 12/20/2020
    Results are reproducible and the kit is easy to follow.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 12/18/2020
    Used for measuring IL2 and IFN-gamma release in T cell cultures.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Hon Wai Koon
    Verified Customer | Posted 12/01/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 11/28/2020
    The DuoSet ELISA Ancillary Reagent Kit 2 was used with the Human TNF-alpha DuoSet ELISA kit (DY210), Human IL-6 DuoSet ELISA (DY206) and Human IL-12/IL-23 p40 DuoSet ELISA (DY124) to detect secreted cytokines in GMCSF/IL4 monocyte-derived human dendritic cells stimulated with LPS (100 ng/ml) for 24 hours.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: APK Inmunometabolismo
    Verified Customer | Posted 11/06/2020
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Evan Williams
    Verified Customer | Posted 10/28/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Joseph Ambrose
    Verified Customer | Posted 10/27/2020
    I use this for several different ELISAs. The duoset has worked well for every ELISA I have run.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/23/2020
    Works well!!!
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/23/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/13/2020
    The Ancillary Kit2 that we purchased for in house assay development of our target proteins worked well. We are regularly using this product which is giving us reproducible results.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/13/2020
    DuoSet ELISA Ancillary Reagent Kit 2 was used in combination with the Human TNF-alpha DuoSet ELISA kit (DY210). Monocytes were stimulated with zymosan (25 μg/ml) for 24 hours. After 24 hours, TNFa was assessed in the supernatant.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Milton Quintanilla
    Verified Customer | Posted 10/07/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Pritha Agarwalla
    Verified Customer | Posted 09/30/2020
    Used for quantifying release of IL2 and IFN gamma
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Robert Berahovich
    Verified Customer | Posted 09/29/2020
    Detection of IFN-g produced by BCMA-specific CAR-T cells in response to BCMA-negative and BCMA-positive hematopoietic cell lines.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/14/2020
    Fantastic kit. Very cost effective compared to buying everything separately. Reagent Diluent can be scarce depending on sample protein concentration.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/05/2020
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 06/08/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 03/17/2020
    VERY SENSITIVE, GOOD KIT
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/23/2020
    Performance and reproducibility of the kit are very good (see graph attached). Also, very good customer service and fast shipment.On the downside, the protocol is tricky to undstand.The information to perform the test is spread on three documents (the protocol, the certificate of analysis and the list of materials), so that pooling this information gives you quite the extra work.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/14/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/14/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/11/2020
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 11/14/2019
    Contains all the reagents needed for the experiments and works well!Well done!
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/29/2019
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/29/2019
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/29/2019
    Good standard curve even for linear form
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/06/2019
    The kit works very well.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Venkateswarlu Kanamarlapudi
    Verified Customer | Posted 10/02/2019
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/23/2019
    we used this kit for developing sandwich ELISA's
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/21/2019
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/13/2019
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/08/2019
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Gigi Ng
    Verified Customer | Posted 07/02/2019
    Contains all the reagents needed and works well, p.s. nice ladder
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Bulbul Ahmed
    Verified Customer | Posted 03/30/2019
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Amnon Berger
    Verified Customer | Posted 03/23/2019
    The perfect companion to DuoSet ELISA kits, everything you need to run five plates.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 03/19/2019
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/14/2019
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Ivan Luzardo
    Verified Customer | Posted 12/11/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/31/2018
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/17/2018
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/09/2018
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 09/11/2018
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 08/08/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: VICTOR FARRE ALINS
    Verified Customer | Posted 07/17/2018
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Lucy Quach
    Verified Customer | Posted 06/21/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Andrada Naghi
    Verified Customer | Posted 06/20/2018
    I used this reagent pack with the Guinea Pig IL1B ELISA from R and D systems and it worked exactly as expected. I even extended the standard by two points and had no problems with the output.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 06/19/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/04/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 04/26/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Leslie Priddy
    Verified Customer | Posted 03/30/2018
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 12/01/2017
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: tusar giri
    Verified Customer | Posted 12/01/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/20/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Maria Yanez
    Verified Customer | Posted 08/03/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 06/16/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/30/2017
    Used for measuring NGF in bovine seminal plasma.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/19/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 05/12/2017
    The plates provided are excellent to use; Reagents provided are sufficient and should be well allocated accordingly with preferably proper estimations for each ELISA run. Image attached indicates a sample of the product in use for ELISA
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: reb whitt
    Verified Customer | Posted 04/25/2017
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 04/12/2017
    DY008 is used with DY1918-05 to perform fibronectin ELISA for cell culture supernatants in ARPE-19 cells stimulated with or without StemXVivo for 1-5 days.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Jonatan Dereke
    Verified Customer | Posted 01/26/2017
    We have thus far used this over 20 kits of DY008 for a range of different DuoSet ELISA kits and they are performing very good. There is little variation between the kits and they are a great compliment to the DuoSet kits. Highly recommended when app,licable.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Adam Guess
    Verified Customer | Posted 01/20/2017
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 01/06/2017
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 11/01/2016
    Every reagent provided.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Anonymous
    Verified Customer | Posted 10/17/2016
    This ancillary kit works well as intended and the descriptions on the spec sheet have improved. It is a reliable kit.The only issue is the volumes given are not very convenient for the DuoSet ELISA kit (at least for DY537). The ELISA requires at least 25 mL of Wash buffer per plate to comfortably use an autowasher and priming the system. However, this kit provides its wash buffer in 21 mL vials and a total of 126 mL for 5 plates when the ELISA would use 125 mL for 5 plates - a very close call that has caused problems.The box it is provided in is more ergonomic now than its previous versions as well.
    DuoSet ELISA Ancillary Reagent Kit 2 DY008
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Jessica Hess
    Verified Customer | Posted 06/03/2016
    This was the first purchased the accessory kit when using the DuoSet ELISA kits. It was perfect, worth every penny and made performing ELISAs that much simpler.
  • DuoSet ELISA Ancillary Reagent Kit 2
    Name: Jonatan Dereke
    Verified Customer | Posted 05/19/2016
    Works very well with all compatible DuoSet kits.
  • DuoSet ELISA Ancillary Reagent Kit 2, 5 Plate
    Name: Anonymous
    Verified Customer | Posted 01/08/2016

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FAQs for DuoSet ELISA Ancillary Reagent Kit 2

  • Can I use distilled water for diluting the Reagent Diluent Concentrate 2?

    R&D systems uses deionized water to dilute Reagent Diluent Concentrate 2. We do not have information on how distilled water may affect the performance.

Product Documents for DuoSet ELISA Ancillary Reagent Kit 2

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.