Human Complement Component C9 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-82139

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Saliva, Urine, Milk, Cell Supernatant (100 uL)

Sensitivity

0.28 ng/mL

Assay Range

0.47 - 30 ng/mL

View all Complement Component C9 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human Complement Component C9 in samples. No significant cross-reactivity or interference between Human Complement Component C9 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6%

Inter-Assay Precision (Precision between assays) CV% < 6%

Recovery for Human Complement Component C9 ELISA Kit (Colorimetric)

Recovery

average = 98% for serum; average = 102% for EDTA plasma; average = 100% for cell culture media

Linearity

Human Complement Component C9 ELISA Kit (Colorimetric)

Scientific Data Images for Human Complement Component C9 ELISA Kit (Colorimetric)

Human Complement Component C9 ELISA Kit (Colorimetric)

ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-82139] -

ELISA: Human Complement Component C9 ELISA Kit (Colorimetric) [NBP2-82139] - Standard Curve Reference

Kit Contents for Human Complement Component C9 ELISA Kit (Colorimetric)

  • Micro ELISA Plate, dismountable (8 wells x 12 strips); Reference Standard (2 vials); Concentrated (100X) Biotinylated Detection Ab (120 uL); Concentrated (100X) HRP Conjugate (120 uL); Reference Standard & Sample Diluent (20 mL); Biotinylated Detection Ab

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement Component C9

C9 is synthesised in the liver and monocytes, and is a plasma protein consisting of a single polypeptide chain of molecular weight 71kDa. Normal plasma concentration is 60mg/L. C9 forms part of the membrane attack complex (MAC) the cytolytic terminal complex of the complement pathways. C9 binds to the membrane associated C5b-8, binding of C9 to C5b-8 leads to the circular polymerisation of 12-18 C9 molecules. This is the basis of the hydrophilic, protein-walled, trans-membrane channel formed by the MAC, which leads to cell lysis and destruction.

Alternate Names

complement component 9, complement component C9

Gene Symbol

C9

Additional Complement Component C9 Products

Product Documents for Human Complement Component C9 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Complement Component C9 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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