Human Collagen IV ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75864
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.47 ng/mL
Assay Range
0.78 - 50 ng/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
This kit recognizes Human COL4 in samples. No significant cross-reactivity or interference between Human COL4 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 5%
Inter-Assay Precision (Precision between assays) CV% < 5.19%
Recovery for Human Collagen IV ELISA Kit (Colorimetric)
Recovery
86-106%
Linearity
Scientific Data Images for Human Collagen IV ELISA Kit (Colorimetric)
ELISA: Human Collagen IV ELISA Kit (Colorimetric) [NBP2-75864] -
ELISA: Human Collagen IV ELISA Kit (Colorimetric) [NBP2-75864] - Standard Curve ReferenceKit Contents for Human Collagen IV ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Collagen IV
Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Alternate Names
BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
Gene Symbol
COL4A1
Additional Collagen IV Products
Product Documents for Human Collagen IV ELISA Kit (Colorimetric)
Product Specific Notices for Human Collagen IV ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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