cGMP Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE003

Key Product Details
Assay Length
Sample Type & Volume Required Per Well
Sensitivity
Assay Range
Product Summary for cGMP Parameter Assay Kit
Product Specifications
Format
Measurement
Detection Method
Conjugate
Reactivity
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Saliva, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 85.2 | 184 | 265 | 80.7 | 188 | 273 |
Standard Deviation | 6.9 | 12.5 | 14.0 | 8.0 | 16.5 | 22.4 |
CV% | 8.1 | 6.8 | 5.3 | 9.9 | 8.8 | 8.2 |
Cell Lysates
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 40 | 40 | 40 |
Mean (pg/mL) | 57.0 | 72.0 | 178 | 52.0 | 67.0 | 195 |
Standard Deviation | 2.2 | 4.0 | 9.5 | 4.0 | 4.4 | 12.7 |
CV% | 3.9 | 5.6 | 5.3 | 7.7 | 6.6 | 6.5 |
Recovery for cGMP Parameter Assay Kit
The recovery of cGMP spiked to levels throughout the range of the assay in various matrices was evaluated.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=4) | 100 | 91-108 |
Cell Lysates (n=1) | 92 | 80-98 |
EDTA Plasma (n=4) | 91 | 74-105 |
Saliva (n=4) | 102 | 80-123 |
Urine (n=4) | 90 | 77-98 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cGMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for cGMP Parameter Assay Kit
Multi-species cGMP ELISA Cell Lysate Standard Curve
Multi-species cGMP ELISA Cell Culture Supernate/Plasma/Saliva/Urine Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: cGMP
Long Name
Product Documents for cGMP Parameter Assay Kit
Product Specific Notices for cGMP Parameter Assay Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.