Human CA15-3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59962

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Key Product Details

Sample Type & Volume Required Per Well

Serum (50 - 100 uL)

Sensitivity

3.8 U/mL

Assay Range

15 U/mL - 250 U/mL

View all CA15-3/MUC-1 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human CA15-3. No significant cross-reactivity or interference between human CA15-3 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 20

Scientific Data Images for Human CA15-3 ELISA Kit (Colorimetric)

Human CA15-3 ELISA Kit (Colorimetric) [NBP2-59962] -

Human CA15-3 ELISA Kit (Colorimetric) [NBP2-59962] -

Standard Curve Reference

Kit Contents for Human CA15-3 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Sample Diluent
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CA15-3/MUC-1

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Long Name

Cancer Antigen 15-3

Alternate Names

CA153, MUC1

Gene Symbol

MUC1

Additional CA15-3/MUC-1 Products

Product Documents for Human CA15-3 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human CA15-3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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