Human Arachidonic Acid ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59872

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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell culture supernates and tissue homogenates (50 - 100 uL)

Sensitivity

69.748 ng/mL

Assay Range

78.125 ng/mL - 5000 ng/mL

View all Arachidonic Acid ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human AA. No significant cross-reactivity or interference between human AA and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images for Human Arachidonic Acid ELISA Kit (Colorimetric)

Human Arachidonic Acid ELISA Kit (Colorimetric) [NBP2-59872] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human Arachidonic Acid ELISA Kit (Colorimetric)

Human Arachidonic Acid ELISA Kit (Colorimetric) [NBP2-59872] -

Human Arachidonic Acid ELISA Kit (Colorimetric) [NBP2-59872] - Standard Curve Reference

Kit Contents for Human Arachidonic Acid ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Arachidonic Acid

The minimum detectable dose of human AA is typically less than 69.748 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human AA concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Alternate Names

506-32-1, 506-32-1 ELISA, AA ELISA, ARA, Omega-6 Fatty Acid

Additional Arachidonic Acid Products

Product Documents for Human Arachidonic Acid ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Arachidonic Acid ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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