Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59879

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

23.43 pg/mL

Assay Range

93.75 pg/mL - 6000 pg/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human AD7C-NTP. No significant cross-reactivity or interference between human AD7C-NTP and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Scientific Data Images

ELISA: Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) [NBP2-59879]
Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric)

Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) [NBP2-59879] -

Human Alzheimer Associated Neuronal Thread Protein ELISA Kit (Colorimetric) [NBP2-59879] - Standard Curve Reference

Kit Contents

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Alzheimer Associated Neuronal Thread Protein

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AD7C-NTP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD7C-NTP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AD7C-NTP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AD7C-NTP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

AD7C-NTP, Alzheimer Associated Protein, Alzheimer NTP, Neural Thread Protein, Neuronal Thread Protein

Additional Alzheimer Associated Neuronal Thread Protein Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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