15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61285

Novus Biologicals, part of Bio-Techne
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NBP2-61285
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Key Product Details

Sample Type & Volume Required Per Well

Plasma, serum, and urine from any species (100 uL)

Sensitivity

36.8 pg/ml

Assay Range

195 - 200,000 pg/ml

View all 15-deoxy-Delta 12, 14-PGJ2 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.2%

Inter-Assay Precision (Precision between assays) CV% < 14.4%

Recovery for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

Recovery

Tissue Culture Media - 97.4%
Human Saliva - 94.4%
Human Urine - 113.9%
Porcine Plasma - 105.0%

Scientific Data Images for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

ELISA: 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric) [NBP2-61285] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

  • Antibody
  • Assay buffer
  • concentrate
  • Conjugate
  • GxR IgG Microtiter plate
  • pNpp Substrate
  • Standard
  • Stop solution
  • Wash buffer

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: 15-deoxy-Delta 12, 14-PGJ2

15-deoxy-12,14-Prostaglandin J2 (15-d-PGJ2) is one of the ultimate dehydration products of PGD2, formed via the intermediate 12-PGJ2. 15-d-PGJ2 is physiologically present in body fluids at concentration of 10-12 to 10-9 M, but increases dramatically under stress such as infection and inflammation. 15-d-PGJ2 exerts both pro- and anti-inflammatory effects in a cell-type and concentration-dependent manner, with many of these effects mediated via binding to the isoform of the peroxisome proliferators-activated receptor (PPAR-y). 15-d-PGJ2 appears to induce HO-1 expression by a PPAR-y-independent mechanism mediated by the generation of reactive oxygen species (ROS).

Alternate Names

15-Deoxy-delta-12,14-prostaglandin J2, 15-deoxy-delta12,14-prostaglandin J2, 15-Deoxy-PGJ2, 15d-PGJ2, 7893-55-8

Additional 15-deoxy-Delta 12, 14-PGJ2 Products

Product Documents for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 15-deoxy-Delta 12, 14-PGJ2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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