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Mouse Erythrocyte Lysing Kit

R&D Systems, part of Bio-Techne | Catalog # WL2000

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WL2000

Key Product Details

Species

Mouse

Product Summary for Mouse Erythrocyte Lysing Kit

Kit Summary

For the removal of erythrocytes (RBC) from splenocyte preparations.

Key Benefits

  • Gently lyses erythrocytes
  • Stabilizes leukocytes for analysis by flow cytometry
  • Maintains natural light scattering and fluorescent staining properties of leukocytes
  • Maintains viability of leukocytes for subsequent tissue culture manipulation
 

Why Remove Erythrocytes?

The lysis of erythrocytes from whole blood is an important initial step in the isolation and analysis of enriched leukocyte preparations. Recovered immune cells can be accurately characterized following red blood cell removal. Lysis of erythrocytes under conditions that do not disrupt lymphocytes or myeloid cells is critical for downstream applications utilizing leukocytes harvested from whole blood.

Reagents Provided

The Mouse Erythrocyte Lysing Kit (Catalog # WL2000) contains the following reagents for the lysis of erythrocytes in splenocyte preparations:

  • M-Lyse Buffer Concentrate (10X)
  • Wash Buffer Concentrate (10X)
  • Fixative Concentrate (10X)

*This kit contains sufficient reagents to process 12.5 x 109 splenocytes.

Stability and Storage

Store all reagents at 20 °C to 25 °C. 

Formulation, Preparation, and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at room temperature. Do not use past expiration date.

Product Documents for Mouse Erythrocyte Lysing Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Erythrocyte Lysing Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including formaldehyde and methanol, which are known to the State of California to cause cancer and reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.
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