NeuroXVivo Rat Motor Neuron Culture Kit
R&D Systems, part of Bio-Techne | Catalog # CDK016
Discontinued Product
CDK016 has been discontinued.
View all Neural Cell Culture Kits products.
Key Product Details
Species
Rat
Product Summary for NeuroXVivo Rat Motor Neuron Culture Kit
Kit Summary
Reagents for the culturing and maturation of primary rat motor neurons in culture.
Key Benefits
- Features an optimized serum-free media for rat motor neuron cultures
- Utilizes R&D Systems® Recombinant BDNF, CNTF, GDNF, and Laminin α4
- Detailed protocol is included
- Ideal for both non-experienced and experienced neural cell culture researchers
Why use NeuroXVivo™ Rat Motor Neuron Culture Kit to Culture Primary Rat Motor Neurons?
Spinal motor neuron cultures are an indispensable model system for studying neuronal development, disease, and regeneration. They have been pivotal in investigating molecular and functional mechanisms underlying motor neuron diseases, such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). The survival and development of motor neurons, both in vivo and in vitro, requires specific combinations of growth factors, adhesion molecules, peptides, and vitamins. The NeuroXVivo™ Rat Motor Neuron Culture Kit provides optimized reagents and a validated protocol to successfully culture primary rat motor neurons in serum-free media. Specifically, this kit contains optimized concentrations of three growth factors, an adhesion substrate, and a neuronal media supplement that are required to support the robust growth and maintenance of rat primary motor neuron cultures.
The NeuroXVivo™ Rat Motor Neuron Culture Kit is a simple and reliable system for culturing primary rat motor neurons for both experienced and non-experienced users. This kit contains enough reagents to prepare 100 mL of Complete Motor Neuron Culture Medium, which is enough to culture one 24-well plate of rat motor neurons plated on Laminin I, Poly-D-Lysine-coated 12 mm glass coverslips.
Kit Contents
This kit contains the following reagents to maintain mouse pluripotent stem cells in culture:
- Recombinant BDNF (1000X)
- Recombinant CNTF (1000X)
- Recombinant GDNF (1000X)
- Recombinant Laminin α4 (1000X)
- Motor Neuron Culture Media Supplement (10X)
- Reconstitution Buffer 1
Stability and Storage
Store unopened kit at ≤-20 °C. Do not use past the kit expiration date.
Limitations
- FOR LABORATORY RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
- The safety and efficacy of this product in diagnostic or other clinical uses has not been established.
- This reagent should not be used beyond the expiration date indicated on the label.
- Results may vary due to variations among cells derived from different donors.
Scientific Data Images for NeuroXVivo Rat Motor Neuron Culture Kit
Depolarization Mediated Intracellular Calcium Response in Cultured Rat Motor Neurons
Embryonic day 15 primary rat motor neurons were cultured for 14 daysin vitrousing this kit. Representative traces of intracellular Ca2+response (F340/F380) in Fura-2 loaded rat motor neurons in culture upon addition of 3 µM Glutamate (yellow), 50 mM Potassium Chloride (blue), or HBSS control (grey).
Rat Motor Neurons Express Motor Neuron-specific Markers and Form an Extensive Network of Neuronal Processes
Embryonic day 15 primary rat motor neurons were cultured for 14 days in vitro using this kit. Cultures were stained for the motor neuron-specific markers, Neurofilament 145kDa (red) and Homeobox 9 (green), using the Anti-MNX1/HLXB9 Antibody (Novus Biologicals®, Catalog # NBP2-24691). Neurons were counterstained with DAPI (blue).Formulation, Preparation, and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Product Documents for NeuroXVivo Rat Motor Neuron Culture Kit
Product Specific Notices for NeuroXVivo Rat Motor Neuron Culture Kit
For research use only
Loading...
Loading...
Loading...
Loading...